Detailed information of TA system TA186739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 419881..420611
• Replicon: chromosome
• Accession: NZ_CP068148
• Organism: Serratia liquefaciens strain FDAARGOS_1081

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A515D591
• Locus tag: I6I38_RS01920
• Protein ID: WP_041415720.1
• Coordinates: 420300..420611 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I38_RS01915
• Protein ID: WP_201895978.1
• Coordinates: 419881..420303 (-)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I38_RS01900	415787..416527	+	741	WP_044553680.1	KDGP aldolase family protein	-
I6I38_RS01905	416679..417896	+	1218	WP_201895977.1	lactonase family protein	-
I6I38_RS01910	417948..419864	+	1917	WP_020831882.1	BglG family transcription antiterminator	-
I6I38_RS01915	419881..420303	-	423	WP_201895978.1	helix-turn-helix domain-containing protein	Antitoxin
I6I38_RS01920	420300..420611	-	312	WP_041415720.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6I38_RS01925	420931..421203	-	273	WP_020831874.1	PTS phosphocarrier protein NPr	-
I6I38_RS01930	421200..422054	-	855	WP_012147065.1	RNase adapter RapZ	-
I6I38_RS01935	422186..422647	-	462	WP_115060613.1	PTS IIA-like nitrogen regulatory protein PtsN	-
I6I38_RS01940	422806..423093	-	288	WP_004948547.1	ribosome hibernation promoting factor	-
I6I38_RS01945	423117..424550	-	1434	WP_020831868.1	RNA polymerase factor sigma-54	-
I6I38_RS01950	424601..425326	-	726	WP_020831867.1	LPS export ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	411352..421203	9851

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12299.20 Da
• Isoelectric Point: 10.4493

>T186739 WP_041415720.1 NZ_CP068148:c420611-420300 [Serratia liquefaciens]
MHIISREPFNEAAIRYPNEAASLNTIYKTLRRGTFHTPEDLKSLFPSLDRMKYKAKWWVIDVGGNHLRILFFASFDTQKI
FVKQIVTHAEYDRLLQQYRRNPK

Nucleotide

• Length: 312 bp

>T186739 NZ_CP089768:5225035-5225403 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GCCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15517.89 Da
• Isoelectric Point: 5.2065

>AT186739 WP_201895978.1 NZ_CP068148:c420303-419881 [Serratia liquefaciens]
MITDALKATHELLRAIPLLGGSTSPQDYRDALALVEHLLETDERHPLIDVLAAKIVEYEDNSPDFAEFNQRIAQLPQGVA
ALSVLMEQYGLSQSDFEKEIGKKSLVSQILSGKRSLTIPHIMALAKRFNLPPALFLPEAR

Nucleotide

• Length: 423 bp

>AT186739 NZ_CP089768:5224817-5225038 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A515D591