Detailed information of TA system TA186720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1022271..1022854
• Replicon: chromosome
• Accession: NZ_CP068147
• Organism: Yersinia enterocolitica strain FDAARGOS_1090

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U7FJS5
• Locus tag: I6I47_RS04690
• Protein ID: WP_005162526.1
• Coordinates: 1022271..1022549 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I47_RS04695
• Protein ID: WP_005162529.1
• Coordinates: 1022549..1022854 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I47_RS04680	1017752..1018699	-	948	WP_005162520.1	trehalose operon repressor TreR	-
I6I47_RS04685	1019471..1022191	+	2721	WP_005162523.1	magnesium-translocating P-type ATPase	-
I6I47_RS04690	1022271..1022549	+	279	WP_005162526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I47_RS04695	1022549..1022854	+	306	WP_005162529.1	HigA family addiction module antidote protein	Antitoxin
I6I47_RS04700	1022926..1024854	-	1929	WP_020282732.1	BglG family transcription antiterminator	-
I6I47_RS04705	1024927..1026168	-	1242	WP_049530292.1	lactonase family protein	-
I6I47_RS04710	1026298..1027038	-	741	WP_020282730.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10694.10 Da
• Isoelectric Point: 7.4011

>T186720 WP_005162526.1 NZ_CP068147:1022271-1022549 [Yersinia enterocolitica]
MIKSWKHKGLQRFFETGSAAGINPQHVKRIRDRLRFIDLAETIEDVNVAGYKLHPLQGEREGIWSVTVSGNWRITFEFTA
GDAFILNYEDYH

Nucleotide

• Length: 279 bp

>T186720 NZ_CP089765:2248319-2249236 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCATCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10934.77 Da
• Isoelectric Point: 6.7158

>AT186720 WP_005162529.1 NZ_CP068147:1022549-1022854 [Yersinia enterocolitica]
MKMHNPPHPGEMIEGILEDLGLGIREFARALNVSPSTVQRLVACKAAISPEMAVKLAAVLGSTPAMWLRLQAAYDLGKAE
ENVDVSNLTKLYKPDPISLHS

Nucleotide

• Length: 306 bp

>AT186720 NZ_CP089765:2247555-2248313 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A7U7FJS5