Detailed information of TA system TA186648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 47251..47770
• Replicon: plasmid unnamed3
• Accession: NZ_CP068117
• Organism: Enterococcus hirae strain FDAARGOS_1125

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: I6I82_RS01195
• Protein ID: WP_135982204.1
• Coordinates: 47492..47770 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: I6I82_RS01190
• Protein ID: WP_135982202.1
• Coordinates: 47251..47505 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I82_RS01175	45046..45444	+	399	WP_135982196.1	hypothetical protein	-
I6I82_RS01180	45654..46061	+	408	WP_135982198.1	hypothetical protein	-
I6I82_RS01185	46341..47009	+	669	WP_135982200.1	hypothetical protein	-
I6I82_RS01190	47251..47505	+	255	WP_135982202.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
I6I82_RS01195	47492..47770	+	279	WP_135982204.1	type II toxin-antitoxin system YafQ family toxin	Toxin
I6I82_RS01200	48779..49306	+	528	WP_168353756.1	PrsW family intramembrane metalloprotease	-
I6I82_RS01205	49874..50098	+	225	WP_135982245.1	hypothetical protein	-
I6I82_RS01210	50115..50459	+	345	WP_135982207.1	hypothetical protein	-
I6I82_RS01215	50896..51996	+	1101	WP_135982209.1	Abi family protein	-
I6I82_RS01220	52082..52701	-	620	Protein_53	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..80453	80453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (2-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 11180.14 Da
• Isoelectric Point: 10.3493

>T186648 WP_135982204.1 NZ_CP068117:47492-47770 [Enterococcus hirae]
MLTIEFTPSFKRDYKRMMKKHYDETEFRKVINLLVKQEQEVLRTKYKDHALQGNWKGFRELHVQSDWLLIYKIDGKKLIL
TLVRTGSHDKIL

Nucleotide

• Length: 279 bp

>T186648 NZ_CP089611:1389598-1390029 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9697.93 Da
• Isoelectric Point: 4.2805

>AT186648 WP_135982202.1 NZ_CP068117:47251-47505 [Enterococcus hirae]
MSRLNIRIDDELKEQAREVYEEIGMDLSTAVIVFLKQSVRERKLPFQPGNEPREDIIARYEAENGITTKVSSVDELMEKL
NADD

Nucleotide

• Length: 255 bp

>AT186648 NZ_CP089611:1389341-1389601 [Mycobacterium tuberculosis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA