Detailed information of TA system TA186646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 37141..37649
• Replicon: plasmid unnamed3
• Accession: NZ_CP068117
• Organism: Enterococcus hirae strain FDAARGOS_1125

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: I6I82_RS01160
• Protein ID: WP_135982191.1
• Coordinates: 37380..37649 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: I6I82_RS01155
• Protein ID: WP_135982189.1
• Coordinates: 37141..37395 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I82_RS01130	33336..34544	+	1209	WP_135982174.1	DUF3991 and TOPRIM domain-containing protein	-
I6I82_RS01135	34555..35718	+	1164	WP_135982176.1	glucosaminidase domain-containing protein	-
I6I82_RS01140	35728..36204	+	477	WP_135982178.1	hypothetical protein	-
I6I82_RS01145	36405..36632	+	228	WP_135982185.1	hypothetical protein	-
I6I82_RS01150	36625..36963	+	339	WP_135982187.1	type II toxin-antitoxin system PemK/MazF family toxin	-
I6I82_RS01155	37141..37395	+	255	WP_135982189.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6I82_RS01160	37380..37649	+	270	WP_135982191.1	Txe/YoeB family addiction module toxin	Toxin
I6I82_RS01165	37701..38459	+	759	WP_135982193.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..80453	80453

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-59)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10413.12 Da
• Isoelectric Point: 9.9106

>T186646 WP_135982191.1 NZ_CP068117:37380-37649 [Enterococcus hirae]
MGFTIEIKKNAKKDLPKLKAAGLHKKFDELIQVIRENPFQTPPPYEKLVGVPYYSRRINIQHRLVYSVDTEENVVVILSI
WSQYERGLN

Nucleotide

• Length: 270 bp

>T186646 NZ_CP089611:c1236030-1235719 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9625.68 Da
• Isoelectric Point: 4.3077

>AT186646 WP_135982189.1 NZ_CP068117:37141-37395 [Enterococcus hirae]
MEVTTPTNARNNIYGLIRHVISDNQPVEIINTKNENESVVMISKSDWNAIQETLYLQNTGVIDQIKRHENKGTEELGEID
WDSL

Nucleotide

• Length: 255 bp

>AT186646 NZ_CP089611:c1236350-1236030 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A