Detailed information of TA system TA186645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 60175..60710
• Replicon: plasmid unnamed2
• Accession: NZ_CP068116
• Organism: Enterococcus hirae strain FDAARGOS_1125

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: R2PQC0
• Locus tag: I6I82_RS00600
• Protein ID: WP_002314366.1
• Coordinates: 60438..60710 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: I6I82_RS00595
• Protein ID: WP_098040900.1
• Coordinates: 60175..60438 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I82_RS00575	57629..57829	+	201	Protein_59	type II toxin-antitoxin system RelE/ParE family toxin	-
I6I82_RS00580	58012..58266	+	255	WP_002290397.1	AbrB family transcriptional regulator	-
I6I82_RS00585	58267..58584	+	318	WP_002290394.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6I82_RS00590	59143..60069	+	927	WP_135982294.1	hypothetical protein	-
I6I82_RS00595	60175..60438	+	264	WP_098040900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6I82_RS00600	60438..60710	+	273	WP_002314366.1	Txe/YoeB family addiction module toxin	Toxin
I6I82_RS00605	60788..61117	+	330	WP_002292678.1	AbrB family transcriptional regulator	-
I6I82_RS00610	61107..61394	+	288	WP_135982292.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
I6I82_RS00615	61680..62537	+	858	WP_005874887.1	AAA family ATPase	-
I6I82_RS00620	62538..63086	+	549	WP_135982290.1	hypothetical protein	-
I6I82_RS00625	63211..64764	-	1554	Protein_69	IS1182 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	bsh	1..140928	140928
	-	flank	IS/Tn	-	-	65282..66241	959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.24 Da
• Isoelectric Point: 9.9724

>T186645 WP_002314366.1 NZ_CP068116:60438-60710 [Enterococcus hirae]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T186645 NZ_CP089611:1172488-1173369 [Mycobacterium tuberculosis]
GTGACCAAGCCCTATTCGTCGCCGCCAACGAACCTGCGCTCACTACGAGATCGGCTCACCCAAGTAGCGGAACGGCAAGG
TGTCGTGTTCGGTCGACTGCAGCGGCATGTCGCGATGATTGTTGTCGCACAGTTCGCGGCCACGCTCACCGACGACACCG
GCGCTCCGCTGCTGTTGGTCAAAGGCGGATCGTCGCTGGAACTGCGCCGGGGAATTCCCGATTCGCGGACCTCCAAAGAC
TTCGACACGGTCGCACGTCGCGATATCGAATTAATCCATGAACAGCTCGCTGACGCGGGCGAGACGGGGTGGGAAGGATT
CACTGCAATCTTCACCGCCCCCGAAGAAATCGATGTTCCTGGTATGCCGGTCAAGCCGCGCCGATTCACCGCCAAGCTGA
GCTACCGAGGCCGGGCTTTCGCAACTGTTCCGATCGAGGTCTCCTCCGTCGAAGCCGGCAATGCCGACCAATTCGACACC
CTCACCTCAGACGCGCTCGGCCTCGTGGGCGTACCCGCAGCAGTCGCCGTACCCTGCATGACCATTCCCTGGCAAATCGC
GCAGAAGCTGCACGCAGTAACTGCCGTGCTCGAAGAACCGAAGGTCAACGACCGCGCTCACGACCTGGTGGACTTGCAGC
TTCTTGAAGGACTGTTGCTCGATGCCGACCTCATGCCGACGCGCAGCGCGTGCATCGCGATATTCGAAGCGCGCGCCCAG
CATCCTTGGCCACCGAGAGTCGCCACGCTGCCGCACTGGCCGCTGATCTATGCAGGTGCGCTGGAGGGGCTTGACCACCT
TGAACTCGCCAGGACGGTCGACGCGGCGGCCCAGGCAGTGCAGCGATTCGTTGCGCGGATTGATCGGGCGACGAAAAGAT
GA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9947.16 Da
• Isoelectric Point: 4.5370

>AT186645 WP_098040900.1 NZ_CP068116:60175-60438 [Enterococcus hirae]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQTGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT186645 NZ_CP089611:1171868-1172491 [Mycobacterium tuberculosis]
TTGTGTGCAAAACCGTATCTAATTGATACGATTGCGCACATGGCTATCTGGGATCGCCTCGTCGAGGTTGCCGCCGAGCA
ACATGGCTACGTCACGACTCGCGATGCGCGAGACATCGGCGTCGACCCTGTGCAGCTCCGCCTCCTAGCGGGGCGCGGAC
GTCTTGAGCGTGTCGGCCGAGGTGTGTACCGGGTGCCCGTGCTGCCGCGTGGTGAGCACGACGATCTCGCAGCCGCAGTG
TCGTGGACTTTGGGGCGTGGCGTTATCTCGCATGAGTCGGCCTTGGCGCTTCATGCCCTCGCTGACGTGAACCCGTCGCG
CATCCATCTCACCGTCCCGCGCAACAACCATCCGCGTGCGGCCGGGGGCGAGCTGTACCGAGTTCACCGCCGCGACCTCC
AGGCAGCCCACGTCACTTCGGTCGACGGAATACCCGTCACGACGGTTGCGCGCACCATCAAAGACTGCGTGAAGACGGGC
ACGGATCCTTATCAGCTTCGGGCCGCGATCGAGCGAGCCGAAGCCGAGGGCACGCTTCGTCGTGGGTCAGCAGCTGAGCT
ACGCGCTGCGCTCGATGAGACCACTGCCGGATTACGCGCTCGGCCGAAGCGAGCATCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A829F704