Detailed information of TA system TA186606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1438147..1438874
• Replicon: chromosome
• Accession: NZ_CP068090
• Organism: Shigella boydii strain FDAARGOS_1139

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q31WV0
• Locus tag: I6I96_RS07115
• Protein ID: WP_000550185.1
• Coordinates: 1438560..1438874 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6I96_RS07110
• Protein ID: WP_000560271.1
• Coordinates: 1438147..1438563 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I96_RS07095	1433507..1434553	+	1047	WP_000695473.1	alpha-glucosidase	-
I6I96_RS07100	1435019..1435858	+	840	Protein_1386	alpha-glucosidase	-
I6I96_RS07105	1436084..1438102	+	2019	WP_000121441.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
I6I96_RS07110	1438147..1438563	-	417	WP_000560271.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
I6I96_RS07115	1438560..1438874	-	315	WP_000550185.1	type II toxin-antitoxin system toxin HigB	Toxin
I6I96_RS07120	1439159..1440295	-	1137	WP_000018685.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
I6I96_RS07125	1440380..1440883	+	504	WP_001295542.1	M48 family metallopeptidase	-
I6I96_RS07130	1440960..1441247	+	288	Protein_1392	vancomycin high temperature exclusion protein	-
I6I96_RS07135	1441301..1441998	+	698	Protein_1393	IS1 family transposase	-
I6I96_RS07140	1442016..1442420	+	405	Protein_1394	YdcF family protein	-
I6I96_RS07145	1442499..1443485	+	987	WP_004986115.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1441495..1441998	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12133.19 Da
• Isoelectric Point: 10.0409

>T186606 WP_000550185.1 NZ_CP068090:c1438874-1438560 [Shigella boydii]
MHLITQKALKDAAEKYPQHKTELVALGNMIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T186606 NZ_CP089610:c2413736-2413419 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15007.47 Da
• Isoelectric Point: 4.4547

>AT186606 WP_000560271.1 NZ_CP068090:c1438563-1438147 [Shigella boydii]
MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT186606 NZ_CP089610:c2413948-2413733 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: Q31WV0