Detailed information of TA system TA186559

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54336..54600
• Replicon: plasmid pXJW9B277-I1
• Accession: NZ_CP068044
• Organism: Escherichia coli strain XJW9B277

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: JJE78_RS25890
• Protein ID: WP_001303307.1
• Coordinates: 54448..54600 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	54336..54398 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJE78_RS25875 (50438)	50438..51508	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
JJE78_RS25880 (51527)	51527..52735	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
- (52915)	52915..52975	-	61	NuclAT_1	-	-
- (52915)	52915..52975	-	61	NuclAT_1	-	-
- (52915)	52915..52975	-	61	NuclAT_1	-	-
- (52915)	52915..52975	-	61	NuclAT_1	-	-
JJE78_RS25885 (53042)	53042..53821	-	780	WP_275450201.1	protein FinQ	-
- (54336)	54336..54398	-	63	NuclAT_0	-	Antitoxin
- (54336)	54336..54398	-	63	NuclAT_0	-	Antitoxin
- (54336)	54336..54398	-	63	NuclAT_0	-	Antitoxin
- (54336)	54336..54398	-	63	NuclAT_0	-	Antitoxin
JJE78_RS25890 (54448)	54448..54600	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
JJE78_RS25895 (54672)	54672..54923	-	252	WP_001291965.1	hypothetical protein	-
- (55310)	55310..55361	-	52	NuclAT_2	-	-
- (55310)	55310..55361	-	52	NuclAT_2	-	-
- (55310)	55310..55361	-	52	NuclAT_2	-	-
- (55310)	55310..55361	-	52	NuclAT_2	-	-
JJE78_RS25900 (55847)	55847..56023	-	177	WP_001054900.1	hypothetical protein	-
JJE78_RS25905 (56232)	56232..56441	-	210	Protein_64	hemolysin expression modulator Hha	-
JJE78_RS25910 (56539)	56539..57153	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
JJE78_RS25915 (57229)	57229..59397	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / rmtB / qepA1	-	1..103093	103093

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T186559 WP_001303307.1 NZ_CP068044:54448-54600 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T186559 NZ_CP089600:c87665-87354 [Klebsiella pneumoniae]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCTGTGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGCGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT186559 NZ_CP068044:c54398-54336 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin