Detailed information of TA system TA186550

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66832..67086
• Replicon: plasmid pXJW9B277-F18
• Accession: NZ_CP068043
• Organism: Escherichia coli strain XJW9B277

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JJE78_RS25200
• Protein ID: WP_001312851.1
• Coordinates: 66832..66981 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	67025..67086 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJE78_RS25165 (62384)	62384..62785	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JJE78_RS25170 (62718)	62718..62975	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JJE78_RS25175 (63068)	63068..63721	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JJE78_RS25180 (64660)	64660..65517	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
JJE78_RS25185 (65510)	65510..65992	-	483	WP_001273588.1	hypothetical protein	-
JJE78_RS26510 (65985)	65985..66032	-	48	WP_229471593.1	hypothetical protein	-
JJE78_RS26515 (66023)	66023..66274	+	252	WP_223195197.1	replication protein RepA	-
JJE78_RS25195 (66291)	66291..66548	-	258	WP_000083845.1	replication regulatory protein RepA	-
JJE78_RS25200 (66832)	66832..66981	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (67025)	67025..67086	+	62	NuclAT_1	-	Antitoxin
- (67025)	67025..67086	+	62	NuclAT_1	-	Antitoxin
- (67025)	67025..67086	+	62	NuclAT_1	-	Antitoxin
- (67025)	67025..67086	+	62	NuclAT_1	-	Antitoxin
JJE78_RS25205 (67342)	67342..67416	-	75	Protein_82	endonuclease	-
JJE78_RS25210 (67662)	67662..67874	-	213	WP_001299730.1	ANR family transcriptional regulator	-
JJE78_RS25215 (68010)	68010..68570	-	561	WP_000139315.1	fertility inhibition protein FinO	-
JJE78_RS25220 (68673)	68673..69533	-	861	WP_000704514.1	alpha/beta hydrolase	-
JJE78_RS25225 (69592)	69592..70338	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / floR / sul2 / aph(4)-Ia / aac(3)-IVa / blaCTX-M-14 / fosA3 / tet(A) / qacE / aadA2 / dfrA12 / blaTEM-1B / sitABCD	iutA / iucD / iucC / iucB / iucA	1..133299	133299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T186550 WP_001312851.1 NZ_CP068043:c66981-66832 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T186550 NZ_CP089598:c2782925-2781603 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCTCTCTCACTGCCGCTGCAGTATGGCAATATCACGG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAA
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTTACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTTTCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT186550 NZ_CP068043:67025-67086 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin