Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2975835..2976337 Replicon chromosome
Accession NZ_CP068041
Organism Escherichia coli strain XJW9B277

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7I6H4L3
Locus tag JJE78_RS14595 Protein ID WP_000767831.1
Coordinates 2975835..2976089 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JJE78_RS14600 Protein ID WP_001259255.1
Coordinates 2976086..2976337 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJE78_RS14565 (2970850) 2970850..2971077 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
JJE78_RS14570 (2971091) 2971091..2972149 - 1059 WP_048217810.1 thiosulfate utilization transporter TsuA/YeeE -
JJE78_RS14575 (2972328) 2972328..2973686 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JJE78_RS14580 (2973676) 2973676..2973738 - 63 WP_010723108.1 membrane protein YoeI -
JJE78_RS14585 (2973953) 2973953..2974882 - 930 WP_048217808.1 LysR substrate-binding domain-containing protein -
JJE78_RS14590 (2974928) 2974928..2975752 - 825 WP_000754737.1 SDR family oxidoreductase -
JJE78_RS14595 (2975835) 2975835..2976089 - 255 WP_000767831.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JJE78_RS14600 (2976086) 2976086..2976337 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JJE78_RS14605 (2976621) 2976621..2976671 + 51 WP_001364200.1 his operon leader peptide -
JJE78_RS14610 (2976817) 2976817..2977716 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
JJE78_RS14615 (2977722) 2977722..2979026 + 1305 WP_001375298.1 histidinol dehydrogenase -
JJE78_RS14620 (2979023) 2979023..2980093 + 1071 WP_000108939.1 histidinol-phosphate transaminase -
JJE78_RS14625 (2980093) 2980093..2981160 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10231.67 Da        Isoelectric Point: 8.8202

>T186541 WP_000767831.1 NZ_CP068041:c2976089-2975835 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTYDSLLIAAC
RYHY

Download         Length: 255 bp

>T186541 NZ_CP089592:c2224367-2224116 [Bacillus subtilis]
ATGAACTTCTCCTTTAGTTCCTACCCATATTATAACATGATCAAGCACATTGCAAACATGAAACGATTCTCATTATGGTT
TACCCATATCACATTCATTGGCTTATTCTTAATGTTTCAACTCATCAAAGATTACTTCAGCAGCGAAGGACAGGCGCTAA
TCAACACAATTTTTGTTGTCACATGTATCATTGCCATATTGTTATGGATCATCTATTGTGTATTCCTTAAACTAAGAAAC
AAGTCACACTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT186541 WP_001259255.1 NZ_CP068041:c2976337-2976086 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT186541 NZ_CP089592:2224345-2224445 [Bacillus subtilis]
TAGGAACTAAAGGAGAAGTTCATTCCCCTTTAGCTTAGCTCTCATCGGCGTATACGTTGGCGTTGTCTCTTTGGTCGGAG
CCGCTTGCGTATACGCTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6H4L3

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References