Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 902462..903156 Replicon chromosome
Accession NZ_CP068041
Organism Escherichia coli strain XJW9B277

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag JJE78_RS04305 Protein ID WP_001263489.1
Coordinates 902758..903156 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JJE78_RS04300 Protein ID WP_000554758.1
Coordinates 902462..902755 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJE78_RS04280 (898094) 898094..898591 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JJE78_RS04285 (898815) 898815..900527 - 1713 Protein_842 flagellar biosynthesis protein FlhA -
JJE78_RS04290 (900499) 900499..901284 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JJE78_RS04295 (901355) 901355..902410 + 1056 WP_001226164.1 DNA polymerase IV -
JJE78_RS04300 (902462) 902462..902755 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JJE78_RS04305 (902758) 902758..903156 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JJE78_RS04310 (903166) 903166..903618 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
JJE78_RS04315 (903936) 903936..904142 + 207 Protein_848 RtcB family protein -
JJE78_RS04320 (904138) 904138..904659 + 522 Protein_849 peptide chain release factor H -
JJE78_RS04325 (904716) 904716..906173 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
JJE78_RS04330 (906434) 906434..906892 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (907488) 907488..907568 + 81 NuclAT_9 - -
- (907488) 907488..907568 + 81 NuclAT_9 - -
- (907488) 907488..907568 + 81 NuclAT_9 - -
- (907488) 907488..907568 + 81 NuclAT_9 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T186528 WP_001263489.1 NZ_CP068041:902758-903156 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T186528 NZ_CP089586:c207918-207823 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT186528 WP_000554758.1 NZ_CP068041:902462-902755 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT186528 NZ_CP089586:207738-207795 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References