Detailed information of TA system TA186522

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-agrB/-
• Location: 105971..106392
• Replicon: plasmid pU90-5
• Accession: NZ_CP068040
• Organism: Escherichia coli strain U90

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: JKF55_RS26605
• Protein ID: WP_001302184.1
• Coordinates: 105971..106129 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	106194..106392 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKF55_RS26565	101125..101421	-	297	WP_021558926.1	hypothetical protein	-
JKF55_RS26570	101445..101651	-	207	WP_042634482.1	hypothetical protein	-
JKF55_RS26575	101721..102080	+	360	Protein_117	hypothetical protein	-
JKF55_RS26580	102318..102476	-	159	WP_042634416.1	type I toxin-antitoxin system Hok family toxin	-
-	102528..102676	-	149	NuclAT_1	-	-
-	102528..102676	-	149	NuclAT_1	-	-
-	102528..102676	-	149	NuclAT_1	-	-
-	102528..102676	-	149	NuclAT_1	-	-
JKF55_RS26585	102652..102927	-	276	WP_077631319.1	hypothetical protein	-
JKF55_RS26590	103007..104578	-	1572	WP_000381443.1	IS66 family transposase	-
JKF55_RS26595	104598..104945	-	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
JKF55_RS26600	104945..105595	-	651	WP_000993956.1	IS66 family insertion sequence hypothetical protein	-
JKF55_RS26605	105971..106129	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	106194..106392	-	199	NuclAT_0	-	Antitoxin
-	106194..106392	-	199	NuclAT_0	-	Antitoxin
-	106194..106392	-	199	NuclAT_0	-	Antitoxin
-	106194..106392	-	199	NuclAT_0	-	Antitoxin
JKF55_RS26610	106404..107123	-	720	WP_000116348.1	plasmid SOS inhibition protein A	-
JKF55_RS26615	107120..107554	-	435	WP_116794657.1	conjugation system SOS inhibitor PsiB	-
JKF55_RS26620	107609..109567	-	1959	WP_000117204.1	ParB/RepB/Spo0J family partition protein	-
JKF55_RS26625	109631..109864	-	234	WP_000005985.1	DUF905 family protein	-
JKF55_RS26630	109926..110465	-	540	WP_000290811.1	single-stranded DNA-binding protein	-
JKF55_RS26635	110491..110697	-	207	WP_000275848.1	hypothetical protein	-
JKF55_RS26640	110767..111193	+	427	Protein_130	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa / hlyC / hlyA / hlyB / hlyD	1..126454	126454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T186522 WP_001302184.1 NZ_CP068040:c106129-105971 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T186522 NZ_CP089584:c1076655-1076404 [Xanthomonas translucens pv. undulosa]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGCGTCGTCAACAACCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTTGAAGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTGCGCAGTCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTCGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT186522 NZ_CP068040:c106392-106194 [Escherichia coli]
TCATACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin