Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4519292..4520091 Replicon chromosome
Accession NZ_CP068035
Organism Escherichia coli strain U90

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag JKF55_RS21875 Protein ID WP_000347251.1
Coordinates 4519627..4520091 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A346GP48
Locus tag JKF55_RS21870 Protein ID WP_063102910.1
Coordinates 4519292..4519627 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF55_RS21855 4515077..4515847 - 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -
JKF55_RS21860 4515863..4517197 - 1335 WP_000599653.1 galactarate/glucarate/glycerate transporter GarP -
JKF55_RS21865 4517572..4519143 + 1572 WP_001273761.1 galactarate dehydratase -
JKF55_RS21870 4519292..4519627 + 336 WP_063102910.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JKF55_RS21875 4519627..4520091 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JKF55_RS21880 4520146..4520955 - 810 WP_000072192.1 aga operon transcriptional regulator AgaR -
JKF55_RS21885 4521204..4522484 + 1281 WP_063102909.1 tagatose-bisphosphate aldolase subunit KbaZ -
JKF55_RS21890 4522507..4522980 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JKF55_RS21895 4522991..4523770 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JKF55_RS21900 4523760..4524638 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JKF55_RS21905 4524656..4525090 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T186515 WP_000347251.1 NZ_CP068035:4519627-4520091 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T186515 NZ_CP089533:22219-22596 [Pseudomonas veronii]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12300.90 Da        Isoelectric Point: 5.1296

>AT186515 WP_063102910.1 NZ_CP068035:4519292-4519627 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGGEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT186515 NZ_CP089533:22577-22912 [Pseudomonas veronii]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A346GP48

References