Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3175373..3175875 Replicon chromosome
Accession NZ_CP068035
Organism Escherichia coli strain U90

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JKF55_RS15380 Protein ID WP_000767829.1
Coordinates 3175373..3175627 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JKF55_RS15385 Protein ID WP_001259255.1
Coordinates 3175624..3175875 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF55_RS15350 3170388..3170615 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
JKF55_RS15355 3170629..3171687 - 1059 WP_023568741.1 transport protein YeeE -
JKF55_RS15360 3171866..3173224 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JKF55_RS15365 3173214..3173276 - 63 WP_010723108.1 membrane protein YoeI -
JKF55_RS15370 3173491..3174420 - 930 WP_000803351.1 LysR family transcriptional regulator -
JKF55_RS15375 3174466..3175290 - 825 WP_000754763.1 SDR family oxidoreductase -
JKF55_RS15380 3175373..3175627 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JKF55_RS15385 3175624..3175875 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JKF55_RS15390 3176157..3176207 + 51 WP_001364200.1 his operon leader peptide -
JKF55_RS15395 3176353..3177252 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
JKF55_RS15400 3177258..3178562 + 1305 WP_021823828.1 histidinol dehydrogenase -
JKF55_RS15405 3178559..3179629 + 1071 WP_064986838.1 histidinol-phosphate transaminase -
JKF55_RS15410 3179629..3180696 + 1068 WP_064986837.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T186508 WP_000767829.1 NZ_CP068035:c3175627-3175373 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T186508 NZ_CP089530:485294-485644 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT186508 WP_001259255.1 NZ_CP068035:c3175875-3175624 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT186508 NZ_CP089530:485007-485288 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References