Detailed information of TA system TA186493

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2356770..2357141
• Replicon: chromosome
• Accession: NZ_CP068035
• Organism: Escherichia coli strain U90

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: JKF55_RS11175
• Protein ID: WP_021500490.1
• Coordinates: 2356947..2357141 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2356770..2356948 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKF55_RS11150	2352725..2354098	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
JKF55_RS11155	2354227..2355162	-	936	WP_001774812.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
JKF55_RS11160	2355214..2356449	-	1236	WP_074149259.1	site-specific integrase	-
JKF55_RS11165	2356451..2356666	-	216	WP_000079604.1	excisionase XisR	-
JKF55_RS11170	2356766..2356954	-	189	WP_001383994.1	DUF1187 family protein	-
-	2356770..2356948	+	179	NuclAT_0	-	Antitoxin
-	2356770..2356948	+	179	NuclAT_0	-	Antitoxin
-	2356770..2356948	+	179	NuclAT_0	-	Antitoxin
-	2356770..2356948	+	179	NuclAT_0	-	Antitoxin
JKF55_RS11175	2356947..2357141	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
JKF55_RS11180	2357205..2358257	-	1053	WP_001004422.1	recombinase RecT	-
JKF55_RS11185	2358269..2361391	-	3123	WP_064986939.1	exodeoxyribonuclease VIII	-
JKF55_RS11190	2361493..2361768	-	276	WP_000632298.1	hypothetical protein	-
JKF55_RS11195	2361843..2362043	-	201	WP_071776730.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2351264..2379446	28182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T186493 WP_021500490.1 NZ_CP068035:c2357141-2356947 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T186493 NZ_CP089509:3983214-3983432 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT186493 NZ_CP068035:2356770-2356948 [Escherichia coli]
GACGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin