Detailed information of TA system TA186456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4989169..4989771
• Replicon: chromosome
• Accession: NZ_CP068018
• Organism: Salmonella enterica strain 1722

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: JI996_RS24605
• Protein ID: WP_001159630.1
• Coordinates: 4989169..4989480 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JI996_RS24610
• Protein ID: WP_000362050.1
• Coordinates: 4989481..4989771 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI996_RS24570 (4984282)	4984282..4984881	+	600	WP_000965695.1	glucose-1-phosphatase	-
JI996_RS24575 (4984875)	4984875..4985747	+	873	WP_000921427.1	virulence factor BrkB family protein	-
JI996_RS24580 (4985744)	4985744..4986181	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
JI996_RS24585 (4986226)	4986226..4987167	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
JI996_RS24590 (4987182)	4987182..4987628	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
JI996_RS24595 (4987625)	4987625..4987936	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
JI996_RS24600 (4988022)	4988022..4988951	-	930	WP_001127703.1	alpha/beta hydrolase	-
JI996_RS24605 (4989169)	4989169..4989480	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
JI996_RS24610 (4989481)	4989481..4989771	+	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
JI996_RS24615 (4989818)	4989818..4990747	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
JI996_RS24620 (4990744)	4990744..4991379	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
JI996_RS24625 (4991376)	4991376..4992278	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T186456 WP_001159630.1 NZ_CP068018:4989169-4989480 [Salmonella enterica]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T186456 NZ_CP089445:c1254059-1253841 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT186456 WP_000362050.1 NZ_CP068018:4989481-4989771 [Salmonella enterica]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT186456 NZ_CP089445:c1254459-1254085 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0R9NS64