Detailed information of TA system TA186406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3646153..3646847
• Replicon: chromosome
• Accession: NZ_CP067994
• Organism: Escherichia coli strain CV601

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: JJT18_RS17805
• Protein ID: WP_001263489.1
• Coordinates: 3646153..3646551 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: JJT18_RS17810
• Protein ID: WP_000554758.1
• Coordinates: 3646554..3646847 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3641741..3641821	-	81	NuclAT_12	-	-
-	3641741..3641821	-	81	NuclAT_12	-	-
-	3641741..3641821	-	81	NuclAT_12	-	-
-	3641741..3641821	-	81	NuclAT_12	-	-
JJT18_RS17780	3642417..3642875	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JJT18_RS17785	3643136..3644593	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
JJT18_RS17790	3644650..3645171	-	522	Protein_3477	peptide chain release factor H	-
JJT18_RS17795	3645167..3645373	-	207	Protein_3478	RtcB family protein	-
JJT18_RS17800	3645691..3646143	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
JJT18_RS17805	3646153..3646551	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JJT18_RS17810	3646554..3646847	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JJT18_RS17815	3646899..3647954	-	1056	WP_001226164.1	DNA polymerase IV	-
JJT18_RS17820	3648025..3648810	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
JJT18_RS17825	3648782..3650494	+	1713	Protein_3484	flagellar biosynthesis protein FlhA	-
JJT18_RS17830	3650718..3651215	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T186406 WP_001263489.1 NZ_CP067994:c3646551-3646153 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T186406 NZ_CP089427:739315-739800 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT186406 WP_000554758.1 NZ_CP067994:c3646847-3646554 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT186406 NZ_CP089427:739025-739318 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3