Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3646153..3646847 Replicon chromosome
Accession NZ_CP067994
Organism Escherichia coli strain CV601

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag JJT18_RS17805 Protein ID WP_001263489.1
Coordinates 3646153..3646551 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JJT18_RS17810 Protein ID WP_000554758.1
Coordinates 3646554..3646847 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3641741..3641821 - 81 NuclAT_12 - -
- 3641741..3641821 - 81 NuclAT_12 - -
- 3641741..3641821 - 81 NuclAT_12 - -
- 3641741..3641821 - 81 NuclAT_12 - -
JJT18_RS17780 3642417..3642875 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
JJT18_RS17785 3643136..3644593 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
JJT18_RS17790 3644650..3645171 - 522 Protein_3477 peptide chain release factor H -
JJT18_RS17795 3645167..3645373 - 207 Protein_3478 RtcB family protein -
JJT18_RS17800 3645691..3646143 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
JJT18_RS17805 3646153..3646551 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JJT18_RS17810 3646554..3646847 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JJT18_RS17815 3646899..3647954 - 1056 WP_001226164.1 DNA polymerase IV -
JJT18_RS17820 3648025..3648810 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JJT18_RS17825 3648782..3650494 + 1713 Protein_3484 flagellar biosynthesis protein FlhA -
JJT18_RS17830 3650718..3651215 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T186406 WP_001263489.1 NZ_CP067994:c3646551-3646153 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T186406 NZ_CP089427:739315-739800 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT186406 WP_000554758.1 NZ_CP067994:c3646847-3646554 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT186406 NZ_CP089427:739025-739318 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References