Detailed information of TA system TA186386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 720438..721131
• Replicon: chromosome
• Accession: NZ_CP067994
• Organism: Escherichia coli strain CV601

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: JJT18_RS03480
• Protein ID: WP_000415584.1
• Coordinates: 720438..720734 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: JJT18_RS03485
• Protein ID: WP_000650107.1
• Coordinates: 720736..721131 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJT18_RS03445	715526..715840	-	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-
JJT18_RS03450	715871..716452	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
JJT18_RS03455	716771..717103	+	333	WP_000917684.1	DUF2645 family protein	-
JJT18_RS03460	717149..718498	-	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
JJT18_RS03465	718495..719154	-	660	WP_001221495.1	two-component system response regulator QseB	-
JJT18_RS03470	719306..719698	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
JJT18_RS03475	719751..720233	+	483	WP_000183505.1	transcriptional regulator	-
JJT18_RS03480	720438..720734	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
JJT18_RS03485	720736..721131	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
JJT18_RS03490	721264..722871	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
JJT18_RS03495	723009..725267	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T186386 WP_000415584.1 NZ_CP067994:720438-720734 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T186386 NZ_CP089407:c3559108-3558851 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT186386 WP_000650107.1 NZ_CP067994:720736-721131 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT186386 NZ_CP089407:c3558840-3558289 [Klebsiella michiganensis]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGTGCCTGCCCGACGCTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTTGGCGGAGAGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGGCGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCACGGGTTTGAGCCAGGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGCGGCGCATAAAGCCTGCTGGATGCTGCAGCCGATTTCGTCT
CAGCCGCTGGGACGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65