Detailed information of TA system TA186361

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40395..40812
• Replicon: plasmid p118
• Accession: NZ_CP067431
• Organism: Escherichia coli O22:H8 strain 154

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JI426_RS28055
• Protein ID: WP_223200913.1
• Coordinates: 40690..40812 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	40395..40605 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI426_RS28020	36080..36607	+	528	WP_000290845.1	single-stranded DNA-binding protein	-
JI426_RS28025	36622..36888	+	267	WP_135247454.1	DUF905 family protein	-
JI426_RS28030	36949..38913	+	1965	WP_135247436.1	ParB/RepB/Spo0J family partition protein	-
JI426_RS28035	38982..39416	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JI426_RS28040	39413..40132	+	720	WP_001276237.1	plasmid SOS inhibition protein A	-
JI426_RS28045	40129..40443	+	315	WP_001562885.1	hypothetical protein	-
-	40392..40605	+	214	NuclAT_0	-	-
-	40392..40605	+	214	NuclAT_0	-	-
-	40392..40605	+	214	NuclAT_0	-	-
-	40392..40605	+	214	NuclAT_0	-	-
-	40395..40605	-	211	-	-	Antitoxin
JI426_RS28050	40593..40745	+	153	Protein_41	DUF5431 family protein	-
JI426_RS28055	40690..40812	+	123	WP_223200913.1	Hok/Gef family protein	Toxin
JI426_RS28060	41113..41410	-	298	Protein_43	hypothetical protein	-
JI426_RS28065	41710..42012	+	303	WP_001272236.1	hypothetical protein	-
JI426_RS28070	42168..42455	+	288	WP_000107526.1	hypothetical protein	-
JI426_RS28075	42573..43394	+	822	WP_001234469.1	DUF932 domain-containing protein	-
JI426_RS28080	43690..44337	-	648	WP_135247439.1	transglycosylase SLT domain-containing protein	-
JI426_RS28085	44613..44996	+	384	WP_001151535.1	conjugal transfer relaxosome DNA-binding protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..118340	118340

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T186361 WP_223200913.1 NZ_CP067431:40690-40812 [Escherichia coli O22:H8]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T186361 NZ_CP089385:4235709-4235927 [Klebsiella variicola subsp. variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin

Nucleotide

• Length: 211 bp

>AT186361 NZ_CP067431:c40605-40395 [Escherichia coli O22:H8]
GTGGACTAGACATGCAGAGGCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACAACACGGT
AACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCAGCACGCTGACCAC
ACTCACTTTCCCTGAAAATAATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Antitoxin