Detailed information of TA system TA186359

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40392..40812
• Replicon: plasmid p118
• Accession: NZ_CP067431
• Organism: Escherichia coli O22:H8 strain 154

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JI426_RS28055
• Protein ID: WP_223200913.1
• Coordinates: 40690..40812 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	40392..40605 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI426_RS28020 (36080)	36080..36607	+	528	WP_000290845.1	single-stranded DNA-binding protein	-
JI426_RS28025 (36622)	36622..36888	+	267	WP_135247454.1	DUF905 family protein	-
JI426_RS28030 (36949)	36949..38913	+	1965	WP_135247436.1	ParB/RepB/Spo0J family partition protein	-
JI426_RS28035 (38982)	38982..39416	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JI426_RS28040 (39413)	39413..40132	+	720	WP_001276237.1	plasmid SOS inhibition protein A	-
JI426_RS28045 (40129)	40129..40443	+	315	WP_001562885.1	hypothetical protein	-
- (40392)	40392..40605	+	214	NuclAT_0	-	Antitoxin
- (40392)	40392..40605	+	214	NuclAT_0	-	Antitoxin
- (40392)	40392..40605	+	214	NuclAT_0	-	Antitoxin
- (40392)	40392..40605	+	214	NuclAT_0	-	Antitoxin
JI426_RS28050 (40593)	40593..40745	+	153	Protein_41	DUF5431 family protein	-
JI426_RS28055 (40690)	40690..40812	+	123	WP_223200913.1	Hok/Gef family protein	Toxin
JI426_RS28060 (41113)	41113..41410	-	298	Protein_43	hypothetical protein	-
JI426_RS28065 (41710)	41710..42012	+	303	WP_001272236.1	hypothetical protein	-
JI426_RS28070 (42168)	42168..42455	+	288	WP_000107526.1	hypothetical protein	-
JI426_RS28075 (42573)	42573..43394	+	822	WP_001234469.1	DUF932 domain-containing protein	-
JI426_RS28080 (43690)	43690..44337	-	648	WP_135247439.1	transglycosylase SLT domain-containing protein	-
JI426_RS28085 (44613)	44613..44996	+	384	WP_001151535.1	conjugal transfer relaxosome DNA-binding protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..118340	118340

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T186359 WP_223200913.1 NZ_CP067431:40690-40812 [Escherichia coli O22:H8]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T186359 NZ_CP089385:c2788590-2787268 [Klebsiella variicola subsp. variicola]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGCACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTACCCGACAGCCCGCAGGTCCGCGATCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTGGGACGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCACCTCGAGGGGTTGCGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCCTACCAGT
CGGATATCCCTCTCGGGATGATTACCGGCCAGGATGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGGGCACTGGTGTATTCCGCAGGGCACCACGCCGACCACGCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCTAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCACTGGCCAGGGAACTGG
GCCTGGCGGTGCCGGAGGCAGAGATTATCACCACGCCGCGGATCCGCGCGCTGGCGGTGACCCGCTTTGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCCTTTGGCATCCCGTCGTCGATGAAATA
TGAATCTGACGGCGGGCCGGGTATTGCGGCAATCATGGCCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCGCAG
ACTTTATGAAATTCATGGTTTTTCAGTGGCTGGTTGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGTGGCAGCTACCGTCTAACGCCGTTCTACGACATCATTTCGGCGTTTCCGGTGCTGGGAGGCACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCAACCGCCAAAGCGGTGAACTTTCCTGGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCGGGCCATTACGGAAAATATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGTTATAG

Antitoxin

Nucleotide

• Length: 214 bp

>AT186359 NZ_CP067431:40392-40605 [Escherichia coli O22:H8]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCTGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin