Detailed information of TA system TA186357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 86950..87661
• Replicon: plasmid pO157
• Accession: NZ_CP067430
• Organism: Escherichia coli O22:H8 strain 154

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: JI426_RS27800
• Protein ID: WP_000162415.1
• Coordinates: 86950..87252 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JI426_RS27805
• Protein ID: WP_000806445.1
• Coordinates: 87323..87661 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI426_RS27775 (JI426_005477)	82633..83538	-	906	WP_001258016.1	recombination-associated protein RdgC	-
JI426_RS27780 (JI426_005478)	83598..84251	-	654	WP_000828869.1	hypothetical protein	-
JI426_RS27785 (JI426_005479)	84248..85228	-	981	WP_000046500.1	hypothetical protein	-
JI426_RS27790 (JI426_005480)	85232..85999	-	768	WP_000203293.1	hypothetical protein	-
JI426_RS27795 (JI426_005481)	85996..86787	-	792	WP_000532307.1	hypothetical protein	-
JI426_RS27800 (JI426_005482)	86950..87252	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JI426_RS27805 (JI426_005483)	87323..87661	+	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
JI426_RS27810 (JI426_005484)	87718..87903	+	186	Protein_99	hypothetical protein	-
JI426_RS27815 (JI426_005485)	87940..88668	+	729	WP_061354292.1	hypothetical protein	-
JI426_RS27820 (JI426_005486)	88702..89895	-	1194	WP_001112629.1	hypothetical protein	-
JI426_RS27825 (JI426_005487)	89888..90217	-	330	WP_249869012.1	baseplate protein	-
JI426_RS27830 (JI426_005489)	90546..91199	-	654	WP_000410954.1	hypothetical protein	-
JI426_RS27835 (JI426_005490)	91545..92396	+	852	WP_249869013.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92972	92972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T186357 WP_000162415.1 NZ_CP067430:86950-87252 [Escherichia coli O22:H8]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T186357 NZ_CP089385:1885524-1885856 [Klebsiella variicola subsp. variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCATTATCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT186357 WP_000806445.1 NZ_CP067430:87323-87661 [Escherichia coli O22:H8]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT186357 NZ_CP089385:1885266-1885523 [Klebsiella variicola subsp. variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3