Detailed information of TA system TA186355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5366787..5367389
• Replicon: chromosome
• Accession: NZ_CP067426
• Organism: Escherichia coli O22:H8 strain 154

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JI426_RS26475
• Protein ID: WP_000897305.1
• Coordinates: 5367078..5367389 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JI426_RS26470
• Protein ID: WP_000356397.1
• Coordinates: 5366787..5367077 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI426_RS26445 (5362713)	5362713..5363615	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JI426_RS26450 (5363612)	5363612..5364247	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JI426_RS26455 (5364244)	5364244..5365173	+	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
JI426_RS26460 (5365503)	5365503..5365745	-	243	WP_001086388.1	protein YiiF	-
JI426_RS26465 (5365964)	5365964..5366182	-	219	WP_001295676.1	CopG family transcriptional regulator	-
JI426_RS26470 (5366787)	5366787..5367077	-	291	WP_000356397.1	NadS family protein	Antitoxin
JI426_RS26475 (5367078)	5367078..5367389	-	312	WP_000897305.1	hypothetical protein	Toxin
JI426_RS26480 (5367618)	5367618..5368526	+	909	WP_001162704.1	alpha/beta hydrolase	-
JI426_RS26485 (5368590)	5368590..5369531	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JI426_RS26490 (5369576)	5369576..5370013	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JI426_RS26495 (5370010)	5370010..5370882	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JI426_RS26500 (5370876)	5370876..5371475	-	600	WP_001315111.1	glucose-1-phosphatase	-
JI426_RS26505 (5371574)	5371574..5372359	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T186355 WP_000897305.1 NZ_CP067426:c5367389-5367078 [Escherichia coli O22:H8]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T186355 NZ_CP089385:407179-407526 [Klebsiella variicola subsp. variicola]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCTGGGCTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGACGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT186355 WP_000356397.1 NZ_CP067426:c5367077-5366787 [Escherichia coli O22:H8]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT186355 NZ_CP089385:407526-407825 [Klebsiella variicola subsp. variicola]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACGTTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7