Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4323232..4323773 Replicon chromosome
Accession NZ_CP067426
Organism Escherichia coli O22:H8 strain 154

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag JI426_RS21540 Protein ID WP_000615982.1
Coordinates 4323495..4323773 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JI426_RS21535 Protein ID WP_000729704.1
Coordinates 4323232..4323492 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI426_RS21510 (4319015) 4319015..4319800 - 786 WP_170992204.1 putative lateral flagellar export/assembly protein LafU -
JI426_RS21515 (4319772) 4319772..4321484 + 1713 Protein_4230 flagellar biosynthesis protein FlhA -
JI426_RS21520 (4321589) 4321589..4321867 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JI426_RS21525 (4321860) 4321860..4322216 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JI426_RS21530 (4322273) 4322273..4323046 - 774 WP_122992790.1 C40 family peptidase -
JI426_RS21535 (4323232) 4323232..4323492 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JI426_RS21540 (4323495) 4323495..4323773 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JI426_RS21545 (4323929) 4323929..4324669 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JI426_RS21550 (4324640) 4324640..4325407 - 768 WP_000333380.1 class II glutamine amidotransferase -
JI426_RS21555 (4325613) 4325613..4326191 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T186352 WP_000615982.1 NZ_CP067426:4323495-4323773 [Escherichia coli O22:H8]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T186352 NZ_CP089383:c5082784-5082401 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGTCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT186352 WP_000729704.1 NZ_CP067426:4323232-4323492 [Escherichia coli O22:H8]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT186352 NZ_CP089383:c5083026-5082784 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References