Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 709684..710483 Replicon chromosome
Accession NZ_CP067426
Organism Escherichia coli O22:H8 strain 154

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JI426_RS03440 Protein ID WP_000347273.1
Coordinates 709684..710148 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JI426_RS03445 Protein ID WP_001307405.1
Coordinates 710148..710483 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI426_RS03410 (704685) 704685..705119 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JI426_RS03415 (705137) 705137..706015 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JI426_RS03420 (706005) 706005..706784 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JI426_RS03425 (706795) 706795..707268 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JI426_RS03430 (707291) 707291..708571 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
JI426_RS03435 (708820) 708820..709629 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
JI426_RS03440 (709684) 709684..710148 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JI426_RS03445 (710148) 710148..710483 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JI426_RS03450 (710632) 710632..712203 - 1572 WP_033817271.1 galactarate dehydratase -
JI426_RS03455 (712578) 712578..713912 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JI426_RS03460 (713928) 713928..714698 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T186332 WP_000347273.1 NZ_CP067426:c710148-709684 [Escherichia coli O22:H8]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T186332 NZ_CP089325:c5202691-5202308 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTATGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT186332 WP_001307405.1 NZ_CP067426:c710483-710148 [Escherichia coli O22:H8]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT186332 NZ_CP089325:c5202933-5202691 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References