Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3746936..3747477 Replicon chromosome
Accession NZ_CP067399
Organism Escherichia coli strain XJW9B263

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag JJF91_RS18320 Protein ID WP_000615983.1
Coordinates 3747199..3747477 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag JJF91_RS18315 Protein ID WP_000729703.1
Coordinates 3746936..3747196 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJF91_RS18295 (3742611) 3742611..3743396 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JJF91_RS18300 (3743368) 3743368..3745080 + 1713 Protein_3611 flagellar biosynthesis protein FlhA -
JJF91_RS18305 (3745304) 3745304..3745801 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JJF91_RS18310 (3745977) 3745977..3746726 - 750 WP_000056849.1 C40 family peptidase -
JJF91_RS18315 (3746936) 3746936..3747196 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JJF91_RS18320 (3747199) 3747199..3747477 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JJF91_RS18325 (3747633) 3747633..3748373 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JJF91_RS18330 (3748344) 3748344..3749111 - 768 WP_000333380.1 class II glutamine amidotransferase -
JJF91_RS18335 (3749317) 3749317..3749895 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T186286 WP_000615983.1 NZ_CP067399:3747199-3747477 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T186286 NZ_CP089313:c1063573-1063046 [Salmonella enterica subsp. enterica serovar Indiana]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTAGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT186286 WP_000729703.1 NZ_CP067399:3746936-3747196 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT186286 NZ_CP089313:c1063860-1063570 [Salmonella enterica subsp. enterica serovar Indiana]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References