Detailed information of TA system TA186285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3740739..3741433
• Replicon: chromosome
• Accession: NZ_CP067399
• Organism: Escherichia coli strain XJW9B263

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: JJF91_RS18280
• Protein ID: WP_001263489.1
• Coordinates: 3740739..3741137 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: JJF91_RS18285
• Protein ID: WP_000554758.1
• Coordinates: 3741140..3741433 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3736327)	3736327..3736407	-	81	NuclAT_11	-	-
- (3736327)	3736327..3736407	-	81	NuclAT_11	-	-
- (3736327)	3736327..3736407	-	81	NuclAT_11	-	-
- (3736327)	3736327..3736407	-	81	NuclAT_11	-	-
JJF91_RS18255 (3737003)	3737003..3737461	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JJF91_RS18260 (3737722)	3737722..3739179	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
JJF91_RS18265 (3739236)	3739236..3739757	-	522	Protein_3604	peptide chain release factor H	-
JJF91_RS18270 (3739753)	3739753..3739959	-	207	Protein_3605	RtcB family protein	-
JJF91_RS18275 (3740277)	3740277..3740729	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
JJF91_RS18280 (3740739)	3740739..3741137	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JJF91_RS18285 (3741140)	3741140..3741433	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JJF91_RS18290 (3741485)	3741485..3742540	-	1056	WP_001226164.1	DNA polymerase IV	-
JJF91_RS18295 (3742611)	3742611..3743396	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
JJF91_RS18300 (3743368)	3743368..3745080	+	1713	Protein_3611	flagellar biosynthesis protein FlhA	-
JJF91_RS18305 (3745304)	3745304..3745801	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T186285 WP_001263489.1 NZ_CP067399:c3741137-3740739 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T186285 NZ_CP089313:859102-859500 [Salmonella enterica subsp. enterica serovar Indiana]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT186285 WP_000554758.1 NZ_CP067399:c3741433-3741140 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT186285 NZ_CP089313:858875-859102 [Salmonella enterica subsp. enterica serovar Indiana]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3