Detailed information of TA system TA186267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 714412..715105
• Replicon: chromosome
• Accession: NZ_CP067399
• Organism: Escherichia coli strain XJW9B263

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: JJF91_RS03445
• Protein ID: WP_000415584.1
• Coordinates: 714412..714708 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: JJF91_RS03450
• Protein ID: WP_000650107.1
• Coordinates: 714710..715105 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJF91_RS03410 (709500)	709500..709814	-	315	WP_000958598.1	putative quinol monooxygenase	-
JJF91_RS03415 (709845)	709845..710426	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
JJF91_RS03420 (710745)	710745..711077	+	333	WP_000917685.1	DUF2645 family protein	-
JJF91_RS03425 (711123)	711123..712472	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
JJF91_RS03430 (712469)	712469..713128	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
JJF91_RS03435 (713280)	713280..713672	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
JJF91_RS03440 (713725)	713725..714207	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
JJF91_RS03445 (714412)	714412..714708	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
JJF91_RS03450 (714710)	714710..715105	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
JJF91_RS03455 (715238)	715238..716845	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
JJF91_RS03460 (716983)	716983..719241	+	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T186267 WP_000415584.1 NZ_CP067399:714412-714708 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T186267 NZ_CP089309:2318167-2318664 [Thiocapsa bogorovii]
GTGACCCTGAGTCCGCCGAGCCCTTTGACCGGCTACCATGATTGCAAAGGCTTTTCATGTGGTCGCAAGGCATTGGACGA
CTGGCTGAAAGGTCGCGCATTGCGGAATCAGTCGAGCGGGGCCTCCCGTACCTTCGTGGTCTGCGACCAGAATCGGGTCA
TCGCCTATTACGCGTTGGCCTCCGGGGCCGTTGCCATGACTGCGGCGACCGGTCGACTGCGACGCAATATGCCCGATCCC
ATTCCCGTGGTCATACTCGCGCGTCTTGCAGTCGATCATTCCCATGGCGGCAGGGGGCTGGGCCGGGCGTTGCTGCAGGA
CGCGGGCTGGAGGGTCCTGAACGCCGCCGACGCGATCGGCATTCGCGGCCTGTTGGTGCATGCCATCGACGCGGATGCCC
GATGCTTCTATCACCATCTCGGCTTCGACTCCTCACCGCTCGACCCCGCGACACTGATGATCAGCCTCAAGGATTTACGC
GCCAGCCTCGCGGACTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT186267 WP_000650107.1 NZ_CP067399:714710-715105 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT186267 NZ_CP089309:2317880-2318170 [Thiocapsa bogorovii]
ATGTCGACTGCTCAAGAATTGGCCGCCAAGCGACAGACGCTCAATCTTCGCATCAAGGCGGAGGAGCGCGACTTGATCGA
CCGTGCCGCACGCATTGCCGGCAAGAACCGCACCGAGTTCATCCTGGACGCGAGCCGGCATGCGGCCGAGGAGACCCTGC
TGGATCAAGCACTGCTGCGCCTGTCTCCCGATGCCTTCGCGGCCTTCGAGGCGCGCCTTGATGCGCCGCCGGCCCCGAAT
ACTCGGCTGCGCAAGACCATGCGGGGCAGACCGCCCTGGGACACCGAGTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65