Detailed information of TA system TA186260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4180420..4181022
• Replicon: chromosome
• Accession: NZ_CP067397
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain LB5000

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: JJB81_RS20280
• Protein ID: WP_001159630.1
• Coordinates: 4180420..4180731 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JJB81_RS20285
• Protein ID: WP_000362050.1
• Coordinates: 4180732..4181022 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB81_RS20245 (4175533)	4175533..4176132	+	600	WP_000965695.1	glucose-1-phosphatase	-
JJB81_RS20250 (4176126)	4176126..4176998	+	873	WP_000921427.1	virulence factor BrkB family protein	-
JJB81_RS20255 (4176995)	4176995..4177432	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
JJB81_RS20260 (4177477)	4177477..4178418	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
JJB81_RS20265 (4178433)	4178433..4178879	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
JJB81_RS20270 (4178876)	4178876..4179187	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
JJB81_RS20275 (4179273)	4179273..4180202	-	930	WP_001127703.1	alpha/beta hydrolase	-
JJB81_RS20280 (4180420)	4180420..4180731	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
JJB81_RS20285 (4180732)	4180732..4181022	+	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
JJB81_RS20290 (4181069)	4181069..4181998	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
JJB81_RS20295 (4181995)	4181995..4182630	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
JJB81_RS20300 (4182627)	4182627..4183529	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T186260 WP_001159630.1 NZ_CP067397:4180420-4180731 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T186260 NZ_CP089309:c1222884-1222624 [Thiocapsa bogorovii]
ATGACTAGACGGCTCGCTTGGACCGAAGAGGCCTGGGCAGACCACGTCTACTGGCAAAGCCAAGACCGCAAGACCCTGAA
GCGCATCAACGCGCTCATCGCCGACGTGCTGCGGACGCCGTTCGAAGGTATCGGCAAACCCGAGGCCTTGCGGGAAAACC
TGAGCGGATTCTGGTCGCGTCGCATCGACCAAGCGAATCGCCTGGTCTACGCCGTCGATACGGAGCAGATCACCATCATT
TCCTGCCATTATCACTATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT186260 WP_000362050.1 NZ_CP067397:4180732-4181022 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT186260 NZ_CP089309:c1223131-1222877 [Thiocapsa bogorovii]
ATGCAAACCATCACCTTCTCCGATGCCCGCAATCAACTGAAGCAAGTGCTTGATCGCGTAGCAGACGACGCGGACTACAC
GGTCATCACCCGTCGAGATGCTGAAGACGCGGTCGTGATGTCCCTCGACAGCTTCAATAGTCTGATGGAGACGGTCCACC
TGCTTCGCTCACCGGCCAACGCCGAGCATCTCGCCCGCTCCATTGCCCAATACCGCCAGGGACAGACCGCCGAGCATCCG
TTGCAGCATGACTAG

Structures

Toxin

• AlphaFold DB: A0A0R9NS64