Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2447387..2448076 Replicon chromosome
Accession NZ_CP067370
Organism Klebsiella pneumoniae strain B9122

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag JI969_RS12075 Protein ID WP_021469727.1
Coordinates 2447387..2447704 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag JI969_RS12080 Protein ID WP_020804705.1
Coordinates 2447780..2448076 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI969_RS12045 (2443089) 2443089..2443598 + 510 WP_020802144.1 GNAT family N-acetyltransferase -
JI969_RS12050 (2443608) 2443608..2444534 + 927 WP_032435210.1 amino acid ABC transporter permease -
JI969_RS12055 (2444518) 2444518..2445297 + 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
JI969_RS12060 (2445336) 2445336..2446187 + 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
JI969_RS12065 (2446265) 2446265..2446882 + 618 WP_032425015.1 glutathione S-transferase family protein -
JI969_RS12070 (2446953) 2446953..2447180 + 228 WP_002906690.1 tautomerase PptA -
JI969_RS12075 (2447387) 2447387..2447704 + 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JI969_RS12080 (2447780) 2447780..2448076 + 297 WP_020804705.1 NadS family protein Antitoxin
JI969_RS12085 (2448155) 2448155..2448601 + 447 WP_032435212.1 hypothetical protein -
JI969_RS12090 (2448642) 2448642..2450258 - 1617 WP_004175961.1 carbohydrate porin -
JI969_RS12095 (2450302) 2450302..2451684 - 1383 WP_004151222.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T186186 WP_021469727.1 NZ_CP067370:2447387-2447704 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

>T186186 NZ_CP089270:c2764413-2764237 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT186186 WP_020804705.1 NZ_CP067370:2447780-2448076 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp

>AT186186 NZ_CP089270:c2764191-2763775 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References