Detailed information of TA system TA186156

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128653..128907
• Replicon: plasmid pTBH7P2
• Accession: NZ_CP067344
• Organism: Escherichia coli strain ECJXMCRH7

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JJB06_RS24875
• Protein ID: WP_001312851.1
• Coordinates: 128758..128907 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	128653..128714 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB06_RS24850 (125401)	125401..126147	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
JJB06_RS24855 (126206)	126206..127066	+	861	WP_000704513.1	alpha/beta hydrolase	-
JJB06_RS24860 (127169)	127169..127729	+	561	WP_000139315.1	fertility inhibition protein FinO	-
JJB06_RS24865 (127865)	127865..128077	+	213	WP_001299730.1	ANR family transcriptional regulator	-
JJB06_RS24870 (128323)	128323..128397	+	75	Protein_147	endonuclease	-
- (128653)	128653..128714	-	62	NuclAT_1	-	Antitoxin
- (128653)	128653..128714	-	62	NuclAT_1	-	Antitoxin
- (128653)	128653..128714	-	62	NuclAT_1	-	Antitoxin
- (128653)	128653..128714	-	62	NuclAT_1	-	Antitoxin
JJB06_RS24875 (128758)	128758..128907	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
JJB06_RS24880 (129191)	129191..129448	+	258	WP_000083845.1	replication regulatory protein RepA	-
JJB06_RS25150 (129465)	129465..129716	-	252	WP_223195197.1	replication protein RepA	-
JJB06_RS25155 (129707)	129707..129754	+	48	WP_229471593.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / floR / blaTEM-1A / mph(A) / dfrA12 / aadA2 / qacE / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..130221	130221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T186156 WP_001312851.1 NZ_CP067344:128758-128907 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T186156 NZ_CP089235:65606-66049 [Klebsiella sp. KPN54798]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT186156 NZ_CP067344:c128714-128653 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin