Detailed information of TA system TA186152

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 107323..107592
• Replicon: plasmid pTBH7P2
• Accession: NZ_CP067344
• Organism: Escherichia coli strain ECJXMCRH7

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: JJB06_RS24740
• Protein ID: WP_001323520.1
• Coordinates: 107476..107592 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	107323..107388 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB06_RS24700	102542..103513	+	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-
JJB06_RS25125	104150..104319	+	170	Protein_109	hypothetical protein	-
JJB06_RS25130	104502..104582	-	81	Protein_110	hypothetical protein	-
JJB06_RS24705	104652..104858	+	207	WP_000275856.1	hypothetical protein	-
JJB06_RS24710	104884..105423	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
JJB06_RS24715	105491..105724	+	234	WP_000005990.1	DUF905 family protein	-
JJB06_RS24720	105752..105949	+	198	Protein_114	hypothetical protein	-
JJB06_RS24725	106004..106438	+	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
JJB06_RS24730	106435..107197	+	763	Protein_116	plasmid SOS inhibition protein A	-
JJB06_RS24735	107166..107354	-	189	WP_001299721.1	hypothetical protein	-
-	107166..107390	+	225	NuclAT_0	-	-
-	107166..107390	+	225	NuclAT_0	-	-
-	107166..107390	+	225	NuclAT_0	-	-
-	107166..107390	+	225	NuclAT_0	-	-
-	107323..107388	-	66	-	-	Antitoxin
JJB06_RS25135	107376..107525	+	150	Protein_118	plasmid maintenance protein Mok	-
JJB06_RS24740	107476..107592	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
JJB06_RS24745	107812..108042	+	231	WP_071586998.1	hypothetical protein	-
JJB06_RS25140	108040..108212	-	173	Protein_121	hypothetical protein	-
JJB06_RS25145	108282..108488	+	207	WP_000547968.1	hypothetical protein	-
JJB06_RS24750	108513..108799	+	287	Protein_123	hypothetical protein	-
JJB06_RS24755	108917..109738	+	822	WP_001234426.1	DUF932 domain-containing protein	-
JJB06_RS24760	110035..110637	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
JJB06_RS24765	110958..111341	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JJB06_RS24770	111528..112217	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / floR / blaTEM-1A / mph(A) / dfrA12 / aadA2 / qacE / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..130221	130221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T186152 WP_001323520.1 NZ_CP067344:107476-107592 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T186152 NZ_CP089234:c2742069-2740747 [Klebsiella sp. KPN54798]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCTCTCTCACTGCCGCTGCAGTATGGCAATATCACGG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAA
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTTACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTTTCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT186152 NZ_CP067344:c107388-107323 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin