Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3695039..3695733 | Replicon | chromosome |
| Accession | NZ_CP067342 | ||
| Organism | Escherichia coli strain ECJXMCRH7 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | JJB06_RS18165 | Protein ID | WP_001263493.1 |
| Coordinates | 3695039..3695437 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | JJB06_RS18170 | Protein ID | WP_000554757.1 |
| Coordinates | 3695440..3695733 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3690699) | 3690699..3690779 | - | 81 | NuclAT_13 | - | - |
| - (3690699) | 3690699..3690779 | - | 81 | NuclAT_13 | - | - |
| - (3690699) | 3690699..3690779 | - | 81 | NuclAT_13 | - | - |
| - (3690699) | 3690699..3690779 | - | 81 | NuclAT_13 | - | - |
| JJB06_RS18135 (3690039) | 3690039..3691283 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| JJB06_RS18140 (3691375) | 3691375..3691833 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| JJB06_RS18145 (3692094) | 3692094..3693551 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| JJB06_RS18150 (3693608) | 3693608..3694129 | - | 522 | Protein_3565 | peptide chain release factor H | - |
| JJB06_RS18155 (3694128) | 3694128..3694331 | - | 204 | Protein_3566 | RtcB family protein | - |
| JJB06_RS18160 (3694577) | 3694577..3695029 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| JJB06_RS18165 (3695039) | 3695039..3695437 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| JJB06_RS18170 (3695440) | 3695440..3695733 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| JJB06_RS18175 (3695785) | 3695785..3696840 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| JJB06_RS18180 (3696911) | 3696911..3697696 | - | 786 | WP_000207564.1 | putative lateral flagellar export/assembly protein LafU | - |
| JJB06_RS18185 (3697668) | 3697668..3699380 | + | 1713 | Protein_3572 | flagellar biosynthesis protein FlhA | - |
| JJB06_RS18190 (3699596) | 3699596..3700093 | - | 498 | WP_000006260.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | fdeC / fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA / tssA / hcp1/tssD1 | 3647873..3721534 | 73661 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T186146 WP_001263493.1 NZ_CP067342:c3695437-3695039 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T186146 NZ_CP089227:5981377-5981646 [Nocardia asteroides]
ATGCCTGAACGCAAGCTGGCCTTCAGCGCCTACGGGTGGGAGACCTACACATCGTGGCTGGTTCGCGACCGCACGGTCCT
AAAAGCGGCCAACAAAGTGATCGCCGCCGCGCTCGCCGACCCATTCGCCGGGATCGGTAAGCCTGAGCCGTTGAAGCACC
ATCTCGCCGGCCACTGGTCGCGCCGGGTCACCCGCGAGCATCGAATGATCTACTACGTCACCGATTCCGAGATCGTTGTC
ATCGCGGTCGGCGGGCACTACGACGAGTAG
ATGCCTGAACGCAAGCTGGCCTTCAGCGCCTACGGGTGGGAGACCTACACATCGTGGCTGGTTCGCGACCGCACGGTCCT
AAAAGCGGCCAACAAAGTGATCGCCGCCGCGCTCGCCGACCCATTCGCCGGGATCGGTAAGCCTGAGCCGTTGAAGCACC
ATCTCGCCGGCCACTGGTCGCGCCGGGTCACCCGCGAGCATCGAATGATCTACTACGTCACCGATTCCGAGATCGTTGTC
ATCGCGGTCGGCGGGCACTACGACGAGTAG
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT186146 WP_000554757.1 NZ_CP067342:c3695733-3695440 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT186146 NZ_CP089227:5981127-5981384 [Nocardia asteroides]
ATGACTGCGCTACCGATTTCCACCGCCCGCCAGAACCTCTTCAAGCTGGTTCAGCAGGTCAACGACGACCACGACACGGT
CACCGTTGTCACCAAGTCGGGCGAGAACGCCGTGCTCGTCGCCGAATCGGATTGGAACTCCATTCAGGAGACGCTGTATG
TTCTCGCCACCCACGGCGGCGTACGACTGCTCGAGTCCGCCCGAGATGCCCGCGAGGGCCGATTCGAGGCACACGAGCTG
ATAGATCCCGATGCCTGA
ATGACTGCGCTACCGATTTCCACCGCCCGCCAGAACCTCTTCAAGCTGGTTCAGCAGGTCAACGACGACCACGACACGGT
CACCGTTGTCACCAAGTCGGGCGAGAACGCCGTGCTCGTCGCCGAATCGGATTGGAACTCCATTCAGGAGACGCTGTATG
TTCTCGCCACCCACGGCGGCGTACGACTGCTCGAGTCCGCCCGAGATGCCCGCGAGGGCCGATTCGAGGCACACGAGCTG
ATAGATCCCGATGCCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|