Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3595278..3596005 Replicon chromosome
Accession NZ_CP067311
Organism Escherichia coli strain 027_SK3

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JJB21_RS17390 Protein ID WP_000550189.1
Coordinates 3595278..3595592 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JJB21_RS17395 Protein ID WP_000560266.1
Coordinates 3595589..3596005 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB21_RS17370 (3591436) 3591436..3592422 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
JJB21_RS17375 (3592501) 3592501..3593193 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
JJB21_RS17380 (3593270) 3593270..3593773 - 504 WP_001333820.1 M48 family metallopeptidase -
JJB21_RS17385 (3593858) 3593858..3594994 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JJB21_RS17390 (3595278) 3595278..3595592 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JJB21_RS17395 (3595589) 3595589..3596005 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JJB21_RS17400 (3596050) 3596050..3598068 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JJB21_RS17405 (3598294) 3598294..3600645 - 2352 WP_024238710.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T186115 WP_000550189.1 NZ_CP067311:3595278-3595592 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T186115 NZ_CP089221:193118-193489 [Mycobacterium intracellulare]
GTGATCCTCGTTGACACATCGATATGGATTGACCATCTGCACGTCGCCGAGCATCGCCTCGTCGGCCGCTTGACCAACGA
CGAAATCGGTTGCCACCAAATGGTGATCGAGGAGCTGGCACTCGGTATGATCAAGCAGCGCGACGACGTACTGAGATTGC
TGTCCCATCTGCGCGAGTTTCCGTTGTTGACGCACGCGGAAATCCTCCATCTCGTTGACCGTCGCCGCTTGTGGGGAAGG
GGATTGAGCGCAATCGACGCCCACCTTCTTGGGTCGGTTGCTGTCGTCGACGGTGCACGGCTTTGGACGAGGGACAAGAA
GTTGAAAGCGGCGTGTAGAGAAGTGGGCGTTGCGATCGTCGATGAGTCGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT186115 WP_000560266.1 NZ_CP067311:3595589-3596005 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT186115 NZ_CP089221:192925-193131 [Mycobacterium intracellulare]
ATGAGGACAACAGTGACCATCGACGATGCCTTACTGGCTCAGGCGGCGGAACTGACCGGCGTGGCGGAGAGTGCGGCGCT
TCTCCGTCAGGGATTGCAGACCCTCATTCGGGTAGAGAGCGCCCGCCGTCTCGCGGCGCTCGGCGGCACGGACCCCAAGG
CGTCGGCCGCCCCGCGGCGGAGGCCGCCGACCCGTGATCCTCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References