186113

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2471700..2472197 Replicon chromosome
Accession NZ_CP067311
Organism Escherichia coli strain 027_SK3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JJB21_RS12110 Protein ID WP_001173343.1
Coordinates 2471700..2471873 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JJB21_RS12115 Protein ID WP_000398619.1
Coordinates 2471901..2472197 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB21_RS12100 (2468370) 2468370..2469827 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
JJB21_RS12105 (2470064) 2470064..2471581 + 1518 WP_001295074.1 lysine--tRNA ligase -
JJB21_RS12110 (2471700) 2471700..2471873 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JJB21_RS12115 (2471901) 2471901..2472197 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JJB21_RS12120 (2472424) 2472424..2472696 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
JJB21_RS12125 (2472708) 2472708..2472938 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
JJB21_RS12130 (2473119) 2473119..2474750 + 1632 WP_001565376.1 sensor histidine kinase -
JJB21_RS12135 (2474747) 2474747..2475466 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2462846..2472197 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T186113 WP_001173343.1 NZ_CP067311:c2471873-2471700 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T186113 NZ_CP089220:1769757-1770164 [Mycobacterium intracellulare]
ATGAGCAACCCGCAGGTGGCCGGAGTGCTCGACACTTCGGTGTTCATCGCGACCGAAAGTGGTCGGCCTCTCGACGCGGC
GCTGATCCCTGATCAAGTTGGCACTACGGTTGTTACCCTCGCGGAACTGCACGTTGGTGTGTTGGCCGCGTCGACTTCCG
ATATTAGAGCGCAGCGCCTGGCGACATTGGAATCCATTGCGGATATCGAAGCGCTGCCGGTCGATGACGATGCGGCCCGG
ATGTGGGCCCGGCTGCGGATCCATCTCGCCGAGACCGGTCAACGCGTCCGGATCAACGATCTGTGGATCGCGGCCATCGC
GGCGTCCCGAGGGCTGCCTGTTGTCACGCAGGACGATGACTTTGCCGTCTTGGATGGTGCGGCCAATTTAACCGTTATCC
GCGTCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT186113 WP_000398619.1 NZ_CP067311:c2472197-2471901 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT186113 NZ_CP089220:1769500-1769760 [Mycobacterium intracellulare]
ATGTCTGAAGTGGCATCTCGTGAGCTGCGCAACGATACGGCTGGGGTGCTGCGGCGGGTTCGGGATGGAGAGGACGTCAC
CATCACTGTCAGTGGCCGACCGGTCGCAGTTCTTACCGCAGTTGTCCCTCAGCGCAGGCGTTGGCTGAGCAAGACGGAGT
TGCTGTCGCGCTTGCCCCGCGTTCAAGCGGATCCGGGATTACGCGAGGACCTTCACGCACTCGCCGGCGACACTACCGAG
GAGCTGGGCCCGATCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References