Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1953725..1954266 Replicon chromosome
Accession NZ_CP067311
Organism Escherichia coli strain 027_SK3

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag JJB21_RS09675 Protein ID WP_000615983.1
Coordinates 1953988..1954266 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag JJB21_RS09670 Protein ID WP_000729703.1
Coordinates 1953725..1953985 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB21_RS09650 (1949400) 1949400..1950185 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
JJB21_RS09655 (1950157) 1950157..1951869 + 1713 Protein_1916 flagellar biosynthesis protein FlhA -
JJB21_RS09660 (1952093) 1952093..1952590 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JJB21_RS09665 (1952766) 1952766..1953515 - 750 WP_208771306.1 C40 family peptidase -
JJB21_RS09670 (1953725) 1953725..1953985 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JJB21_RS09675 (1953988) 1953988..1954266 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JJB21_RS09680 (1954422) 1954422..1955162 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JJB21_RS09685 (1955133) 1955133..1955900 - 768 WP_000333380.1 class II glutamine amidotransferase -
JJB21_RS09690 (1956106) 1956106..1956684 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1910962..1954266 43304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T186112 WP_000615983.1 NZ_CP067311:1953988-1954266 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T186112 NZ_CP089220:748939-749235 [Mycobacterium intracellulare]
GTGAAGCGGTATGTACTTTCGCCCGCCGCCCGCGCCGACCTGGAACAGATCTGGGATTACACCTGCCAGCGCTGGGATGA
CGATCAGGCCGAGGAATACGTCCGTGAAATCCAGCGCGCGATCGAACGAGTGGTCGACAATCCTATGATCGGACGGGCCT
GCGACGAACTCCGCCCTGGCTACCGGAAGCACGCTGTTGGATCGCACACGCTCTACTACCGGATTGTCACCGGCGACGTG
ATCAATGTGGTGCGCATACTCCACCAACGGATGGATGTCGACCGGCACCTCGACTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT186112 WP_000729703.1 NZ_CP067311:1953725-1953985 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT186112 NZ_CP089220:748691-748942 [Mycobacterium intracellulare]
GTGGGCAAGAATACGTCCTTCAGCCTCGACGAGCATTACAGCGCATTCATCGATGAGGAGGTGGCGTCGGGCCGTTACCG
CTCGGCCAGTGACGTCGTGCGTTCTGCTCTGCGGCTGCTTGAGGATCGTGAGACCCGCCTGCGCGCATTGCGCCAGGCAC
TCGACGCCGGCGAACGAAGTGGTGAGCCGACACCATTCGACTTCGATGAGTTCGTTGCGCGCAAGCGAGCTGAGCAACCT
CGCCGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References