Detailed information of TA system TA186111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1947528..1948222
• Replicon: chromosome
• Accession: NZ_CP067311
• Organism: Escherichia coli strain 027_SK3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: JJB21_RS09635
• Protein ID: WP_001263493.1
• Coordinates: 1947528..1947926 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JJB21_RS09640
• Protein ID: WP_000554757.1
• Coordinates: 1947929..1948222 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (1943188)	1943188..1943268	-	81	NuclAT_8	-	-
- (1943188)	1943188..1943268	-	81	NuclAT_8	-	-
- (1943188)	1943188..1943268	-	81	NuclAT_8	-	-
- (1943188)	1943188..1943268	-	81	NuclAT_8	-	-
JJB21_RS09605 (1942528)	1942528..1943772	-	1245	WP_000189541.1	esterase FrsA	-
JJB21_RS09610 (1943864)	1943864..1944322	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JJB21_RS09615 (1944583)	1944583..1946040	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
JJB21_RS09620 (1946097)	1946097..1946618	-	522	Protein_1909	peptide chain release factor H	-
JJB21_RS09625 (1946617)	1946617..1946820	-	204	Protein_1910	RtcB family protein	-
JJB21_RS09630 (1947066)	1947066..1947518	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JJB21_RS09635 (1947528)	1947528..1947926	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JJB21_RS09640 (1947929)	1947929..1948222	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JJB21_RS09645 (1948274)	1948274..1949329	-	1056	WP_001226164.1	DNA polymerase IV	-
JJB21_RS09650 (1949400)	1949400..1950185	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
JJB21_RS09655 (1950157)	1950157..1951869	+	1713	Protein_1916	flagellar biosynthesis protein FlhA	-
JJB21_RS09660 (1952093)	1952093..1952590	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1910962..1954266	43304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T186111 WP_001263493.1 NZ_CP067311:c1947926-1947528 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T186111 NZ_CP089220:575381-575752 [Mycobacterium intracellulare]
GTGATCCTCGTTGACACATCGATATGGATTGACCATCTGCACGTCGCCGAGCAGCGCCTCGTCGGCCGCTTGACCAACGA
CGAAATCGGTTGCCACCAAATGGTGATCGAGGAGCTGGCACTCGGTATGATCAAGCAGCGCGACGACGTACTGAGATTGC
TGTCCCATCTGCGCGAGTTTCCGTTGTTGACGCACGCGGAAATCCTCCATCTCGTTGACCGTCGCCGCTTGTGGGGAAGG
GGATTGAGTGCAATCGACGCCCACCTTCTTGGGTCGGTTGCTGTCGTCGACGGTGCACGGCTTTGGACGAGGGACAAGAA
GTTGAAAGCGGCGTGTAAAGAAGTGGGTGTTGCGATCGTCGATGAGTCGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT186111 WP_000554757.1 NZ_CP067311:c1948222-1947929 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT186111 NZ_CP089220:575188-575394 [Mycobacterium intracellulare]
ATGAGGACAACAGTGACCATCGACGATGCCTTACTGGCTCAGGCGGCGGAACTGACCGGCGTGACGGAGAGTGCGGCGCT
TCTCCGTCAGGGATTGCAGACCCTCATTCGGGTAGAGAGCGCCCGCCGTCTCGCGGCGCTCGGCGGCACGGACCCCAAGG
CGTCGGCCGCCCCGCGGCGGAGGCCGCCGACCCGTGATCCTCGTTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6