Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4367651..4368450 Replicon chromosome
Accession NZ_CP067307
Organism Escherichia coli strain ABW_A19

Toxin (Protein)


Gene name yhaV Uniprot ID A0A246NUP1
Locus tag JJB22_RS20610 Protein ID WP_021577991.1
Coordinates 4367986..4368450 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JJB22_RS20605 Protein ID WP_001307405.1
Coordinates 4367651..4367986 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB22_RS20590 (4363436) 4363436..4364206 - 771 WP_001058233.1 2-dehydro-3-deoxyglucarate aldolase -
JJB22_RS20595 (4364222) 4364222..4365556 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JJB22_RS20600 (4365931) 4365931..4367502 + 1572 WP_001273756.1 galactarate dehydratase -
JJB22_RS20605 (4367651) 4367651..4367986 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JJB22_RS20610 (4367986) 4367986..4368450 + 465 WP_021577991.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JJB22_RS20615 (4368505) 4368505..4369314 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JJB22_RS20620 (4369563) 4369563..4370843 + 1281 WP_021577992.1 tagatose-bisphosphate aldolase subunit KbaZ -
JJB22_RS20625 (4370866) 4370866..4371339 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JJB22_RS20630 (4371350) 4371350..4372129 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JJB22_RS20635 (4372119) 4372119..4372997 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JJB22_RS20640 (4373015) 4373015..4373449 + 435 WP_000948826.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T186093 WP_021577991.1 NZ_CP067307:4367986-4368450 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKILAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T186093 NZ_CP089216:c3069737-3069306 [Mycobacterium kansasii]
ATGCATTGCGTTGACGTCAACGTTCTCGTCAACGCCCATCGAGCCGACCTGCGAGAACACCCGAACTACCGAGATGTACT
CGAGCGGCTGGCCAACGGCGACGAGCCGCTCGGTCTGCCGGACAGCGTGCTCGCCGGCTTCGTCCGGGTGGTGACCAACC
GCCGCATCTTCGTCGAACCGACCAGCCAGCCCGACGCATGGCAGGCGGTGGATGCCTTGCTCGCGGCACCCGCCGCGATA
CGGCTCAAGCCCGGCGAGCGGCACTGGACGCTGTTTCGGCAGCTCGCCGCCGATATCGATGCGAGTGGCAACGACATCGC
GGACGCGTATCTGGCCGCCTACGCCCTGGAGAACAACGCGACCTGGGTGAGCGCAGACCGCGGTTTCGCCCGCTTTGGCC
GGCTGCGCTGGCGTCATCCGCTGGATATGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT186093 WP_001307405.1 NZ_CP067307:4367651-4367986 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT186093 NZ_CP089216:c3069981-3069724 [Mycobacterium kansasii]
GTGCGCACGACGATCCGGATCGACGACGAGCTCTACCGCGAGGTGAAGGCACGGGCCGCTCGATCCGGGCGTACCGTGGC
CGCGGTTCTCGAAGATGCGGTGCGATGCGGTCTCAATCCGCCCGGGCAGTCGGCGGCCGGGCGTTATCGAGTCCAGCCGA
TTGGCAGCGGCGGGCTTAGACCCGGCGTGGATCTGTCGTCGAACGCCGCGGTCGCAGAGGCGATGGACGAGGGCGTGCCG
GTCGATGCATTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A246NUP1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References