Detailed information of TA system TA186071

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2185494..2185865
• Replicon: chromosome
• Accession: NZ_CP067307
• Organism: Escherichia coli strain ABW_A19

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: JJB22_RS10120
• Protein ID: WP_001317028.1
• Coordinates: 2185671..2185865 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2185494..2185672 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB22_RS10090 (2181246)	2181246..2181419	+	174	WP_001296046.1	protein YnaL	-
JJB22_RS10095 (2181449)	2181449..2182822	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
JJB22_RS10100 (2182951)	2182951..2183886	-	936	WP_080030171.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
JJB22_RS10105 (2183938)	2183938..2185173	-	1236	WP_000040852.1	site-specific integrase	-
JJB22_RS10110 (2185175)	2185175..2185390	-	216	WP_000079604.1	excisionase XisR	-
- (2185494)	2185494..2185672	+	179	NuclAT_0	-	Antitoxin
- (2185494)	2185494..2185672	+	179	NuclAT_0	-	Antitoxin
- (2185494)	2185494..2185672	+	179	NuclAT_0	-	Antitoxin
- (2185494)	2185494..2185672	+	179	NuclAT_0	-	Antitoxin
JJB22_RS10115 (2185469)	2185469..2185678	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
JJB22_RS10120 (2185671)	2185671..2185865	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
JJB22_RS10125 (2185922)	2185922..2186731	-	810	WP_000166319.1	recombination protein RecT	-
JJB22_RS10130 (2186724)	2186724..2187548	-	825	Protein_1999	PD-(D/E)XK nuclease-like domain-containing protein	-
JJB22_RS10135 (2187584)	2187584..2189335	-	1752	Protein_2000	Tn3-like element TnAs1 family transposase	-
JJB22_RS10140 (2189339)	2189339..2189731	+	393	WP_001351729.1	isochorismatase family cysteine hydrolase	-
JJB22_RS10145 (2189869)	2189869..2190753	+	885	WP_000058717.1	EamA family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T186071 WP_001317028.1 NZ_CP067307:c2185865-2185671 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T186071 NZ_CP089202:800922-801179 [Vibrio splendidus]
ATGAATATTGTTTCTTTTACTGAGGCAAGAAATAGCCTTAAGTCTGTGCTCGATAGAGTGGTAGACGATTCTGATGTTAC
GGTAATAACCAGACGAGATTCAGAAGATGCCGTGGTAATGTCTCTTGACCATTTTAATTCAATGCAAGAAACCTTGTACC
TAATGAGCTCGCCTAAAAACGCTGAAAGATTAGCTGAATCCATTGCTCAACTCGAAGCCGGAAATACAACCGTTCGAGAA
TTGAATAATGACGAATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT186071 NZ_CP067307:2185494-2185672 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin