Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 926467..927008 Replicon chromosome
Accession NZ_CP067307
Organism Escherichia coli strain ABW_A19

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag JJB22_RS04230 Protein ID WP_000615980.1
Coordinates 926467..926745 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JJB22_RS04235 Protein ID WP_000729704.1
Coordinates 926748..927008 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB22_RS04215 (924049) 924049..924627 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
JJB22_RS04220 (924833) 924833..925600 + 768 WP_000333380.1 class II glutamine amidotransferase -
JJB22_RS04225 (925571) 925571..926311 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JJB22_RS04230 (926467) 926467..926745 - 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JJB22_RS04235 (926748) 926748..927008 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JJB22_RS04240 (927218) 927218..927967 + 750 WP_000056850.1 C40 family peptidase -
JJB22_RS04245 (928143) 928143..928640 + 498 WP_033815252.1 REP-associated tyrosine transposase RayT -
JJB22_RS04250 (928873) 928873..930585 - 1713 Protein_830 flagellar biosynthesis protein FlhA -
JJB22_RS04255 (930557) 930557..931342 + 786 WP_000207555.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - vgrG/tssI / rhs/PAAR / gmhA/lpcA 912119..958197 46078


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T186067 WP_000615980.1 NZ_CP067307:c926745-926467 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T186067 NZ_CP089165:1197867-1198229 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT186067 WP_000729704.1 NZ_CP067307:c927008-926748 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT186067 NZ_CP089165:1197700-1197870 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References