Detailed information of TA system TA186060

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 100947..101373
• Replicon: plasmid pABW_A32a
• Accession: NZ_CP067304
• Organism: Escherichia coli strain ABW_A32

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JJB23_RS22635
• Protein ID: WP_001372321.1
• Coordinates: 100947..101072 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	101149..101373 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB23_RS22610 (96524)	96524..97126	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
JJB23_RS22615 (97423)	97423..98244	-	822	WP_001234426.1	DUF932 domain-containing protein	-
JJB23_RS22620 (98362)	98362..98649	-	288	WP_000107535.1	hypothetical protein	-
JJB23_RS22625 (98900)	98900..99880	+	981	WP_032154316.1	IS110 family transposase	-
JJB23_RS23565 (99997)	99997..100257	-	261	WP_042046747.1	hypothetical protein	-
JJB23_RS23570 (100327)	100327..100499	+	173	Protein_116	hypothetical protein	-
JJB23_RS22630 (100497)	100497..100727	-	231	WP_071586998.1	hypothetical protein	-
JJB23_RS22635 (100947)	100947..101072	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JJB23_RS23575 (101014)	101014..101163	-	150	Protein_119	plasmid maintenance protein Mok	-
- (101149)	101149..101373	-	225	NuclAT_0	-	Antitoxin
- (101149)	101149..101373	-	225	NuclAT_0	-	Antitoxin
- (101149)	101149..101373	-	225	NuclAT_0	-	Antitoxin
- (101149)	101149..101373	-	225	NuclAT_0	-	Antitoxin
JJB23_RS22640 (101185)	101185..101373	+	189	WP_001299721.1	hypothetical protein	-
JJB23_RS22645 (101342)	101342..102104	-	763	Protein_121	plasmid SOS inhibition protein A	-
JJB23_RS22650 (102101)	102101..102535	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
JJB23_RS22655 (102590)	102590..102787	-	198	Protein_123	hypothetical protein	-
JJB23_RS22660 (102815)	102815..103048	-	234	WP_000005987.1	DUF905 family protein	-
JJB23_RS22665 (103116)	103116..103655	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
JJB23_RS22670 (103681)	103681..103887	-	207	WP_000275856.1	hypothetical protein	-
JJB23_RS23580 (103957)	103957..104037	+	81	Protein_127	hypothetical protein	-
JJB23_RS23585 (104220)	104220..104389	-	170	Protein_128	hypothetical protein	-
JJB23_RS22675 (105026)	105026..105997	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-65 / aac(3)-IId / sitABCD	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..126378	126378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T186060 WP_001372321.1 NZ_CP067304:c101072-100947 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T186060 NZ_CP089162:1399856-1400044 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT186060 NZ_CP067304:c101373-101149 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin