Detailed information of TA system TA186055

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37055..37309
• Replicon: plasmid pABW_A32a
• Accession: NZ_CP067304
• Organism: Escherichia coli strain ABW_A32

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JJB23_RS22285
• Protein ID: WP_001312851.1
• Coordinates: 37055..37204 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	37248..37309 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB23_RS22250 (32607)	32607..33008	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JJB23_RS22255 (32941)	32941..33198	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JJB23_RS22260 (33291)	33291..33944	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JJB23_RS22265 (34883)	34883..35740	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
JJB23_RS22270 (35733)	35733..36215	-	483	WP_001273588.1	hypothetical protein	-
JJB23_RS23550 (36208)	36208..36255	-	48	WP_229471593.1	hypothetical protein	-
JJB23_RS23555 (36246)	36246..36497	+	252	WP_223195197.1	replication protein RepA	-
JJB23_RS22280 (36514)	36514..36771	-	258	WP_000083845.1	replication regulatory protein RepA	-
JJB23_RS22285 (37055)	37055..37204	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (37248)	37248..37309	+	62	NuclAT_1	-	Antitoxin
- (37248)	37248..37309	+	62	NuclAT_1	-	Antitoxin
- (37248)	37248..37309	+	62	NuclAT_1	-	Antitoxin
- (37248)	37248..37309	+	62	NuclAT_1	-	Antitoxin
JJB23_RS22290 (37565)	37565..37639	-	75	Protein_47	endonuclease	-
JJB23_RS22295 (37885)	37885..38097	-	213	WP_001299730.1	ANR family transcriptional regulator	-
JJB23_RS22300 (38233)	38233..38793	-	561	WP_000139315.1	fertility inhibition protein FinO	-
JJB23_RS22305 (38896)	38896..39756	-	861	WP_000704513.1	alpha/beta hydrolase	-
JJB23_RS22310 (39815)	39815..40561	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-65 / aac(3)-IId / sitABCD	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..126378	126378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T186055 WP_001312851.1 NZ_CP067304:c37204-37055 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T186055 NZ_CP089162:89417-89626 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT186055 NZ_CP067304:37248-37309 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin