Detailed information of TA system TA186049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2599759..2600261
• Replicon: chromosome
• Accession: NZ_CP067303
• Organism: Escherichia coli strain ABW_A32

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: JJB23_RS12605
• Protein ID: WP_000767829.1
• Coordinates: 2599759..2600013 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: JJB23_RS12610
• Protein ID: WP_001259255.1
• Coordinates: 2600010..2600261 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB23_RS12575 (2594774)	2594774..2595001	-	228	WP_000985272.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
JJB23_RS12580 (2595015)	2595015..2596073	-	1059	WP_001759318.1	thiosulfate utilization transporter TsuA/YeeE	-
JJB23_RS12585 (2596252)	2596252..2597610	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
JJB23_RS12590 (2597600)	2597600..2597662	-	63	WP_010723108.1	membrane protein YoeI	-
JJB23_RS12595 (2597877)	2597877..2598806	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
JJB23_RS12600 (2598852)	2598852..2599676	-	825	WP_000754737.1	SDR family oxidoreductase	-
JJB23_RS12605 (2599759)	2599759..2600013	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
JJB23_RS12610 (2600010)	2600010..2600261	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
JJB23_RS12615 (2600545)	2600545..2600595	+	51	WP_001364200.1	his operon leader peptide	-
JJB23_RS12620 (2600741)	2600741..2601640	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
JJB23_RS12625 (2601646)	2601646..2602950	+	1305	WP_074514295.1	histidinol dehydrogenase	-
JJB23_RS12630 (2602947)	2602947..2604017	+	1071	WP_000108933.1	histidinol-phosphate transaminase	-
JJB23_RS12635 (2604017)	2604017..2605084	+	1068	WP_208787003.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T186049 WP_000767829.1 NZ_CP067303:c2600013-2599759 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T186049 NZ_CP089161:c407065-406799 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT186049 WP_001259255.1 NZ_CP067303:c2600261-2600010 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT186049 NZ_CP089161:c407322-407065 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0