186035

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 858097..858791 Replicon chromosome
Accession NZ_CP067303
Organism Escherichia coli strain ABW_A32

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag JJB23_RS04075 Protein ID WP_001263489.1
Coordinates 858393..858791 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag JJB23_RS04070 Protein ID WP_000554758.1
Coordinates 858097..858390 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB23_RS04050 (853729) 853729..854226 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JJB23_RS04055 (854450) 854450..856162 - 1713 Protein_797 flagellar biosynthesis protein FlhA -
JJB23_RS04060 (856134) 856134..856919 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JJB23_RS04065 (856990) 856990..858045 + 1056 WP_001226164.1 DNA polymerase IV -
JJB23_RS04070 (858097) 858097..858390 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
JJB23_RS04075 (858393) 858393..858791 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
JJB23_RS04080 (858801) 858801..859253 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
JJB23_RS04085 (859571) 859571..859777 + 207 Protein_803 RtcB family protein -
JJB23_RS04090 (859773) 859773..860294 + 522 Protein_804 peptide chain release factor H -
JJB23_RS04095 (860351) 860351..861808 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
JJB23_RS04100 (862069) 862069..862527 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (863123) 863123..863203 + 81 NuclAT_11 - -
- (863123) 863123..863203 + 81 NuclAT_11 - -
- (863123) 863123..863203 + 81 NuclAT_11 - -
- (863123) 863123..863203 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T186035 WP_001263489.1 NZ_CP067303:858393-858791 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T186035 NZ_CP089157:c2256374-2256186 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT186035 WP_000554758.1 NZ_CP067303:858097-858390 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT186035 NZ_CP089157:c2256663-2256403 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References