Detailed information of TA system TA186028

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 131970..132396
• Replicon: plasmid pABW_A33a
• Accession: NZ_CP067300
• Organism: Escherichia coli strain ABW_A33

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JJB24_RS22980
• Protein ID: WP_001372321.1
• Coordinates: 131970..132095 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	132172..132396 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB24_RS22960 (127559)	127559..128149	+	591	WP_001376243.1	transglycosylase SLT domain-containing protein	-
JJB24_RS22965 (128446)	128446..129267	-	822	WP_001234426.1	DUF932 domain-containing protein	-
JJB24_RS22970 (129385)	129385..129672	-	288	WP_000107535.1	hypothetical protein	-
JJB24_RS22975 (129923)	129923..130903	+	981	WP_032154316.1	IS110 family transposase	-
JJB24_RS23895 (131020)	131020..131280	-	261	WP_042046747.1	hypothetical protein	-
JJB24_RS23900 (131350)	131350..131522	+	173	Protein_154	hypothetical protein	-
JJB24_RS23905 (131520)	131520..131750	-	231	WP_071586998.1	hypothetical protein	-
JJB24_RS22980 (131970)	131970..132095	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JJB24_RS23910 (132037)	132037..132186	-	150	Protein_157	plasmid maintenance protein Mok	-
- (132172)	132172..132396	-	225	NuclAT_0	-	Antitoxin
- (132172)	132172..132396	-	225	NuclAT_0	-	Antitoxin
- (132172)	132172..132396	-	225	NuclAT_0	-	Antitoxin
- (132172)	132172..132396	-	225	NuclAT_0	-	Antitoxin
JJB24_RS22985 (132208)	132208..132396	+	189	WP_001299721.1	hypothetical protein	-
JJB24_RS22990 (132365)	132365..133127	-	763	Protein_159	plasmid SOS inhibition protein A	-
JJB24_RS22995 (133124)	133124..133558	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
JJB24_RS23000 (133613)	133613..133810	-	198	Protein_161	hypothetical protein	-
JJB24_RS23005 (133838)	133838..134071	-	234	WP_000005987.1	DUF905 family protein	-
JJB24_RS23010 (134139)	134139..134678	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
JJB24_RS23015 (134704)	134704..134910	-	207	WP_000275856.1	hypothetical protein	-
JJB24_RS23915 (134980)	134980..135060	+	81	Protein_165	hypothetical protein	-
JJB24_RS23920 (135243)	135243..135412	-	170	Protein_166	hypothetical protein	-
JJB24_RS23020 (136049)	136049..137020	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-65 / aac(3)-IId / sitABCD	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..157401	157401

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T186028 WP_001372321.1 NZ_CP067300:c132095-131970 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T186028 NZ_CP089156:c685457-685248 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGATTATTAATATAATAGACGGTCAGGTAACTGTAGCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT186028 NZ_CP067300:c132396-132172 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin