Detailed information of TA system TA186020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3683953..3684646
• Replicon: chromosome
• Accession: NZ_CP067299
• Organism: Escherichia coli strain ABW_A33

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: JJB24_RS17665
• Protein ID: WP_000415584.1
• Coordinates: 3684350..3684646 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: JJB24_RS17660
• Protein ID: WP_000650107.1
• Coordinates: 3683953..3684348 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB24_RS17650 (3679817)	3679817..3682075	-	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-
JJB24_RS17655 (3682213)	3682213..3683820	-	1608	WP_074520052.1	ABC transporter substrate-binding protein	-
JJB24_RS17660 (3683953)	3683953..3684348	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
JJB24_RS17665 (3684350)	3684350..3684646	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
JJB24_RS17670 (3684851)	3684851..3685333	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
JJB24_RS17675 (3685386)	3685386..3685778	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
JJB24_RS17680 (3685930)	3685930..3686589	+	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
JJB24_RS17685 (3686586)	3686586..3687935	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
JJB24_RS17690 (3687981)	3687981..3688313	-	333	WP_000917685.1	DUF2645 family protein	-
JJB24_RS17695 (3688632)	3688632..3689213	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
JJB24_RS17700 (3689244)	3689244..3689558	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T186020 WP_000415584.1 NZ_CP067299:c3684646-3684350 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T186020 NZ_CP089154:c270569-270108 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTACTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGTTTATCAGATAGAGAAAAAGCAGGAATTCTCTTCGAAGAAT
TAGCGCATAATAAACTTACATACGGTGATATAGCCGATTACTCGAATTTCAACAATCGCAAGTTCGAAAATTACGCAAGA
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTTGAAGCGCACAATTACGGTGTACGTAATTTATATGAGTT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCTATTACATTTGAGCCGTTGAGAGTTTTTAAATATAAGGAAATATAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT186020 WP_000650107.1 NZ_CP067299:c3684348-3683953 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT186020 NZ_CP089154:c270905-270582 [Staphylococcus aureus]
ATGAGAACTTCAGCGGAAATAGGTAAATTAATCAAACAACTACGAAAAGAGAATAATGTGAATTTAACTGATTTTGCAAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATATGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAGA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAAACAGAAAACGAAGTACAACATCGTGCA
GCTCATTTAGAAGGAGAATTAACTGATGACGAGTGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAA
GTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65