Detailed information of TA system TA186017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2622937..2623439
• Replicon: chromosome
• Accession: NZ_CP067299
• Organism: Escherichia coli strain ABW_A33

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: JJB24_RS12790
• Protein ID: WP_000767829.1
• Coordinates: 2622937..2623191 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: JJB24_RS12795
• Protein ID: WP_001259255.1
• Coordinates: 2623188..2623439 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB24_RS12760 (2617952)	2617952..2618179	-	228	WP_000985272.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
JJB24_RS12765 (2618193)	2618193..2619251	-	1059	WP_001759318.1	thiosulfate utilization transporter TsuA/YeeE	-
JJB24_RS12770 (2619430)	2619430..2620788	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
JJB24_RS12775 (2620778)	2620778..2620840	-	63	WP_010723108.1	membrane protein YoeI	-
JJB24_RS12780 (2621055)	2621055..2621984	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
JJB24_RS12785 (2622030)	2622030..2622854	-	825	WP_000754737.1	SDR family oxidoreductase	-
JJB24_RS12790 (2622937)	2622937..2623191	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
JJB24_RS12795 (2623188)	2623188..2623439	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
JJB24_RS12800 (2623723)	2623723..2623773	+	51	WP_001364200.1	his operon leader peptide	-
JJB24_RS12805 (2623919)	2623919..2624818	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
JJB24_RS12810 (2624824)	2624824..2626128	+	1305	WP_074514295.1	histidinol dehydrogenase	-
JJB24_RS12815 (2626125)	2626125..2627195	+	1071	WP_000108933.1	histidinol-phosphate transaminase	-
JJB24_RS12820 (2627195)	2627195..2628262	+	1068	WP_208787003.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T186017 WP_000767829.1 NZ_CP067299:c2623191-2622937 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T186017 NZ_CP089154:84664-84873 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT186017 WP_001259255.1 NZ_CP067299:c2623439-2623188 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT186017 NZ_CP089154:84359-84652 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAAAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0