Detailed information of TA system TA186003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 858097..858791
• Replicon: chromosome
• Accession: NZ_CP067299
• Organism: Escherichia coli strain ABW_A33

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: JJB24_RS04070
• Protein ID: WP_001263489.1
• Coordinates: 858393..858791 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: JJB24_RS04065
• Protein ID: WP_000554758.1
• Coordinates: 858097..858390 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB24_RS04045 (853729)	853729..854226	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
JJB24_RS04050 (854450)	854450..856162	-	1713	Protein_797	flagellar biosynthesis protein FlhA	-
JJB24_RS04055 (856134)	856134..856919	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
JJB24_RS04060 (856990)	856990..858045	+	1056	WP_001226164.1	DNA polymerase IV	-
JJB24_RS04065 (858097)	858097..858390	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JJB24_RS04070 (858393)	858393..858791	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JJB24_RS04075 (858801)	858801..859253	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
JJB24_RS04080 (859571)	859571..859777	+	207	Protein_803	RtcB family protein	-
JJB24_RS04085 (859773)	859773..860294	+	522	Protein_804	peptide chain release factor H	-
JJB24_RS04090 (860351)	860351..861808	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
JJB24_RS04095 (862069)	862069..862527	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (863123)	863123..863203	+	81	NuclAT_11	-	-
- (863123)	863123..863203	+	81	NuclAT_11	-	-
- (863123)	863123..863203	+	81	NuclAT_11	-	-
- (863123)	863123..863203	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T186003 WP_001263489.1 NZ_CP067299:858393-858791 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T186003 NZ_CP089142:c1866076-1865702 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT186003 WP_000554758.1 NZ_CP067299:858097-858390 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT186003 NZ_CP089142:c1866533-1866165 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3