Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 852053..852594 Replicon chromosome
Accession NZ_CP067299
Organism Escherichia coli strain ABW_A33

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag JJB24_RS04030 Protein ID WP_000615983.1
Coordinates 852053..852331 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag JJB24_RS04035 Protein ID WP_000729703.1
Coordinates 852334..852594 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB24_RS04015 (849635) 849635..850213 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
JJB24_RS04020 (850419) 850419..851186 + 768 WP_000333380.1 class II glutamine amidotransferase -
JJB24_RS04025 (851157) 851157..851897 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JJB24_RS04030 (852053) 852053..852331 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JJB24_RS04035 (852334) 852334..852594 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JJB24_RS04040 (852804) 852804..853553 + 750 WP_000056849.1 C40 family peptidase -
JJB24_RS04045 (853729) 853729..854226 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JJB24_RS04050 (854450) 854450..856162 - 1713 Protein_797 flagellar biosynthesis protein FlhA -
JJB24_RS04055 (856134) 856134..856919 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 825081..858045 32964


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T186002 WP_000615983.1 NZ_CP067299:c852331-852053 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T186002 NZ_CP089142:1854179-1854433 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT186002 WP_000729703.1 NZ_CP067299:c852594-852334 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT186002 NZ_CP089142:1853931-1854182 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References