Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3611761..3612488 Replicon chromosome
Accession NZ_CP067265
Organism Escherichia coli strain ABW_S38

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JJB13_RS17500 Protein ID WP_000550189.1
Coordinates 3611761..3612075 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JJB13_RS17505 Protein ID WP_000560266.1
Coordinates 3612072..3612488 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB13_RS17480 (3607919) 3607919..3608905 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
JJB13_RS17485 (3608984) 3608984..3609676 - 693 WP_208779952.1 vancomycin high temperature exclusion protein -
JJB13_RS17490 (3609753) 3609753..3610256 - 504 WP_001333820.1 M48 family metallopeptidase -
JJB13_RS17495 (3610341) 3610341..3611477 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JJB13_RS17500 (3611761) 3611761..3612075 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JJB13_RS17505 (3612072) 3612072..3612488 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JJB13_RS17510 (3612533) 3612533..3614551 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JJB13_RS17515 (3614777) 3614777..3617128 - 2352 WP_024238710.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T185993 WP_000550189.1 NZ_CP067265:3611761-3612075 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T185993 NZ_CP089138:32395-32646 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT185993 WP_000560266.1 NZ_CP067265:3612072-3612488 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT185993 NZ_CP089138:32633-32860 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References