185991

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2471798..2472295 Replicon chromosome
Accession NZ_CP067265
Organism Escherichia coli strain ABW_S38

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JJB13_RS12110 Protein ID WP_001173343.1
Coordinates 2471798..2471971 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JJB13_RS12115 Protein ID WP_000398619.1
Coordinates 2471999..2472295 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB13_RS12100 (2468468) 2468468..2469925 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
JJB13_RS12105 (2470162) 2470162..2471679 + 1518 WP_001295074.1 lysine--tRNA ligase -
JJB13_RS12110 (2471798) 2471798..2471971 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JJB13_RS12115 (2471999) 2471999..2472295 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JJB13_RS12120 (2472522) 2472522..2472794 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
JJB13_RS12125 (2472806) 2472806..2473036 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
JJB13_RS12130 (2473217) 2473217..2474848 + 1632 WP_001565376.1 sensor histidine kinase -
JJB13_RS12135 (2474845) 2474845..2475564 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2462944..2472295 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T185991 WP_001173343.1 NZ_CP067265:c2471971-2471798 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T185991 NZ_CP089137:84899-85048 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT185991 WP_000398619.1 NZ_CP067265:c2472295-2471999 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT185991 NZ_CP089137:c84854-84795 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References