Detailed information of TA system TA185989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1947625..1948319
• Replicon: chromosome
• Accession: NZ_CP067265
• Organism: Escherichia coli strain ABW_S38

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: JJB13_RS09635
• Protein ID: WP_001263493.1
• Coordinates: 1947625..1948023 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JJB13_RS09640
• Protein ID: WP_000554757.1
• Coordinates: 1948026..1948319 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (1943285)	1943285..1943365	-	81	NuclAT_8	-	-
- (1943285)	1943285..1943365	-	81	NuclAT_8	-	-
- (1943285)	1943285..1943365	-	81	NuclAT_8	-	-
- (1943285)	1943285..1943365	-	81	NuclAT_8	-	-
JJB13_RS09605 (1942625)	1942625..1943869	-	1245	WP_000189541.1	esterase FrsA	-
JJB13_RS09610 (1943961)	1943961..1944419	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JJB13_RS09615 (1944680)	1944680..1946137	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
JJB13_RS09620 (1946194)	1946194..1946715	-	522	Protein_1909	peptide chain release factor H	-
JJB13_RS09625 (1946714)	1946714..1946917	-	204	Protein_1910	RtcB family protein	-
JJB13_RS09630 (1947163)	1947163..1947615	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JJB13_RS09635 (1947625)	1947625..1948023	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JJB13_RS09640 (1948026)	1948026..1948319	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JJB13_RS09645 (1948371)	1948371..1949426	-	1056	WP_001226164.1	DNA polymerase IV	-
JJB13_RS09650 (1949497)	1949497..1950282	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
JJB13_RS09655 (1950254)	1950254..1951966	+	1713	Protein_1916	flagellar biosynthesis protein FlhA	-
JJB13_RS09660 (1952190)	1952190..1952687	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1911058..1954363	43305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T185989 WP_001263493.1 NZ_CP067265:c1948023-1947625 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T185989 NZ_CP089135:114655-115071 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT185989 WP_000554757.1 NZ_CP067265:c1948319-1948026 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT185989 NZ_CP089135:114428-114658 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6